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Generation of melanoma-specific, cytotoxic CD4(+) T helper 2 cells: requirement of both HLA-DR15 and Fas antigens on melanomas for their lysis by Th2 cells.

Publication ,  Journal Article
Zennadi, R; Abdel-Wahab, Z; Seigler, HF; Darrow, TL
Published in: Cell Immunol
June 15, 2001

Recognition of melanoma antigens by HLA class-II-restricted CD4(+) T lymphocytes has been investigated. Two cytotoxic CD4(+) T cell lines were established by stimulating PBLs from a melanoma patient with either parental or IFN-gamma-transduced autologous tumor cells. These T cells secreted IL-4, but not IL-2, IFN-gamma, or TNF-beta, in response to the autologous melanoma cells, suggesting that they belong to the Th2 subtype. Their cytotoxicity was directed against the IFN-gamma-transduced melanoma cells and was HLA-DR-restricted. The autologous and two allogeneic IFN-gamma-modified melanoma cell lines shared melanoma antigen(s) presented in the context of HLA-DR15. HLA-DR15(+) nonmelanoma cells were resistant targets indicating that the shared antigen(s) is melanoma associated. Parental autologous and HLA-DR-matched allogeneic melanoma cell lines, displaying low levels of HLA-DR antigens, induced Th2 proliferation and cytokine release, but were insensitive to lysis prior to upregulation of HLA-DR and Fas antigens by IFN-gamma. Cytolysis was inhibited by anti-HLA-DR and by anti-Fas antibodies, suggesting that the cytolysis is mediated via the Fas pathway. While small amounts of HLA-DR15 molecules on melanoma cells are sufficient for Th2 proliferation and cytokine release, higher amounts of HLA-DR15 and the expression of Fas are required for CD4(+)-mediated lysis.

Duke Scholars

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

June 15, 2001

Volume

210

Issue

2

Start / End Page

96 / 105

Location

Netherlands

Related Subject Headings

  • fas Receptor
  • Tumor Necrosis Factor-alpha
  • Tumor Cells, Cultured
  • Transfection
  • Th2 Cells
  • T-Lymphocytes, Cytotoxic
  • Polymorphism, Single-Stranded Conformational
  • Polymerase Chain Reaction
  • Melanoma
  • Lymphokines
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Zennadi, R., Abdel-Wahab, Z., Seigler, H. F., & Darrow, T. L. (2001). Generation of melanoma-specific, cytotoxic CD4(+) T helper 2 cells: requirement of both HLA-DR15 and Fas antigens on melanomas for their lysis by Th2 cells. Cell Immunol, 210(2), 96–105. https://doi.org/10.1006/cimm.2001.1809
Zennadi, R., Z. Abdel-Wahab, H. F. Seigler, and T. L. Darrow. “Generation of melanoma-specific, cytotoxic CD4(+) T helper 2 cells: requirement of both HLA-DR15 and Fas antigens on melanomas for their lysis by Th2 cells.Cell Immunol 210, no. 2 (June 15, 2001): 96–105. https://doi.org/10.1006/cimm.2001.1809.
Zennadi, R., et al. “Generation of melanoma-specific, cytotoxic CD4(+) T helper 2 cells: requirement of both HLA-DR15 and Fas antigens on melanomas for their lysis by Th2 cells.Cell Immunol, vol. 210, no. 2, June 2001, pp. 96–105. Pubmed, doi:10.1006/cimm.2001.1809.
Journal cover image

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

June 15, 2001

Volume

210

Issue

2

Start / End Page

96 / 105

Location

Netherlands

Related Subject Headings

  • fas Receptor
  • Tumor Necrosis Factor-alpha
  • Tumor Cells, Cultured
  • Transfection
  • Th2 Cells
  • T-Lymphocytes, Cytotoxic
  • Polymorphism, Single-Stranded Conformational
  • Polymerase Chain Reaction
  • Melanoma
  • Lymphokines