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Transduction of human melanoma cells with the gamma interferon gene enhances cellular immunity.

Publication ,  Journal Article
Abdel-Wahab, ZA; Osanto, S; Darrow, TL; Barber, JR; Vervaert, CE; Gangavalli, R; McCallister, TJ; Seigler, HF
Published in: Cancer Gene Ther
September 1994

Human tumor cells transduced with the gamma interferon (gamma IFN) gene are currently used in specific active immunotherapy protocols to enhance the antitumor immune responses of cancer patients. This in vitro study was undertaken to examine the initial events in the cellular immune response that may occur following the administration of the gamma IFN-transduced cell vaccine. Human melanoma tumor cell lines were transduced with a MoMLV-based retroviral vector carrying the human gamma IFN gene. The transduced cells expressed the cytokine gene, secreted biologically active gamma IFN, and exhibited enhanced expression of MHC class I and class II (HLA-DR), and ICAM-1 surface antigens. The gamma IFN-transduced and corresponding parental melanoma cells were used for the induction of short-term lymphocyte cultures. Peripheral blood lymphocytes or lymph node cells from 20 melanoma patients were stimulated for 5 to 15 days with autologous or MHC class I-matched allogeneic parental or gamma IFN-transduced melanoma cells. Seven of the 20 lymphocyte cultures showed substantial increases in lytic activity following stimulation with the transduced melanoma cells in comparison to control lymphocyte cultures stimulated with unmodified parental melanoma. The cytolytic activity stimulated with gamma IFN-modified melanomas was mediated partly by MHC-restricted cytotoxic T lymphocytes and partly by NK cells. Lymphocyte cultures that displayed increases in cytotoxicity after stimulation with the gamma IFN-transduced melanoma cells also exhibited enhanced expression or induction of one or more of the following lymphokines: IL-4, IL-1 alpha, IL-1 beta, gamma IFN, and TNF-alpha.(ABSTRACT TRUNCATED AT 250 WORDS)

Duke Scholars

Published In

Cancer Gene Ther

ISSN

0929-1903

Publication Date

September 1994

Volume

1

Issue

3

Start / End Page

171 / 179

Location

England

Related Subject Headings

  • Vaccines
  • Tumor Cells, Cultured
  • Transfection
  • T-Lymphocytes, Cytotoxic
  • Recombinant Proteins
  • Oncology & Carcinogenesis
  • Molecular Sequence Data
  • Melanoma
  • Lymphokines
  • Lymphocyte Activation
 

Citation

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MLA
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Abdel-Wahab, Z. A., Osanto, S., Darrow, T. L., Barber, J. R., Vervaert, C. E., Gangavalli, R., … Seigler, H. F. (1994). Transduction of human melanoma cells with the gamma interferon gene enhances cellular immunity. Cancer Gene Ther, 1(3), 171–179.
Abdel-Wahab, Z. A., S. Osanto, T. L. Darrow, J. R. Barber, C. E. Vervaert, R. Gangavalli, T. J. McCallister, and H. F. Seigler. “Transduction of human melanoma cells with the gamma interferon gene enhances cellular immunity.Cancer Gene Ther 1, no. 3 (September 1994): 171–79.
Abdel-Wahab ZA, Osanto S, Darrow TL, Barber JR, Vervaert CE, Gangavalli R, et al. Transduction of human melanoma cells with the gamma interferon gene enhances cellular immunity. Cancer Gene Ther. 1994 Sep;1(3):171–9.
Abdel-Wahab, Z. A., et al. “Transduction of human melanoma cells with the gamma interferon gene enhances cellular immunity.Cancer Gene Ther, vol. 1, no. 3, Sept. 1994, pp. 171–79.
Abdel-Wahab ZA, Osanto S, Darrow TL, Barber JR, Vervaert CE, Gangavalli R, McCallister TJ, Seigler HF. Transduction of human melanoma cells with the gamma interferon gene enhances cellular immunity. Cancer Gene Ther. 1994 Sep;1(3):171–179.

Published In

Cancer Gene Ther

ISSN

0929-1903

Publication Date

September 1994

Volume

1

Issue

3

Start / End Page

171 / 179

Location

England

Related Subject Headings

  • Vaccines
  • Tumor Cells, Cultured
  • Transfection
  • T-Lymphocytes, Cytotoxic
  • Recombinant Proteins
  • Oncology & Carcinogenesis
  • Molecular Sequence Data
  • Melanoma
  • Lymphokines
  • Lymphocyte Activation