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Generation of primary tumor-specific cytotoxic T lymphocytes from autologous and human lymphocyte antigen class I-matched allogeneic peripheral blood lymphocytes by B7 gene-modified melanoma cells.

Publication ,  Journal Article
Yang, S; Darrow, TL; Seigler, HF
Published in: Cancer Res
April 15, 1997

Expression of B7.1 costimulatory molecules on tumor cells has been shown to elicit antitumor immunity in mice. In the present study, we have developed a human B7.1 retroviral vector system to effectively transduce human melanoma cell lines and investigated the potential role of B7.1 in the generation of tumor-specific CTLs from peripheral blood lymphocytes (PBLs) in vitro. We have demonstrated that B7.1-modified melanoma cells are able to induce primary CTL activity from autologous, human lymphocyte antigen (HLA) class I-matched allogeneic PBLs and purified CD8+ T cells in the absence of exogenous cytokines. CTLs generated by B7.1 are tumor specific and HLA class I restricted, and CD8+ T cells are primarily responsible for this specific cytotoxicity. Furthermore, CTLs generated from HLA class I-matched PBLs by B7.1 are cytolytic to tumor cells autologous to the stimulated PBLs. These data suggest that B7.1-modified tumor cells can be used as a potent tumor vaccine for both autologous and HLA class I-matched allogeneic patients.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

April 15, 1997

Volume

57

Issue

8

Start / End Page

1561 / 1568

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • T-Lymphocytes, Cytotoxic
  • Skin Neoplasms
  • Oncology & Carcinogenesis
  • Mice
  • Melanoma
  • Immunophenotyping
  • Humans
  • Histocompatibility Antigens Class I
 

Published In

Cancer Res

ISSN

0008-5472

Publication Date

April 15, 1997

Volume

57

Issue

8

Start / End Page

1561 / 1568

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • T-Lymphocytes, Cytotoxic
  • Skin Neoplasms
  • Oncology & Carcinogenesis
  • Mice
  • Melanoma
  • Immunophenotyping
  • Humans
  • Histocompatibility Antigens Class I