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Recognition and lysis of human melanoma by a CD3+, CD4+, CD8- T-cell clone restricted by HLA-A2.

Publication ,  Journal Article
Darrow, TL; Abdel-Wahab, Z; Quinn-Allen, MA; Seigler, HF
Published in: Cell Immunol
August 25, 1996

The in vitro cytotoxic response to human melanoma is characterized by CD3+ CD8+ T-cells which recognize shared peptide antigens presented in the context of HLA class-I-encoded gene products. We report here studies of a CD3+, CD4+, CD8-, HLA-A2-restricted, melanoma-specific cytotoxic T-cell clone derived by limiting dilution from a T-cell line induced in PBLs from a melanoma patient following in vitro stimulation with an HLA-A2-matched melanoma cell line. The CD4+ cytotoxic T-cell clone is lytic only for melanomas which share the HLA-A2 allele, and the cytotoxicity is blocked by antibody to the T-cell receptor and by antibody to HLA class I. The clone proliferates only following stimulation with HLA-A2-matched melanoma tumor cells. The data suggest that cytotoxic CD4+ T-cells may play a significant role in immunity to melanoma, and HLA class-I-restricted recognition of melanoma may not necessarily require the CD8 molecule on the lytic T-cell.

Duke Scholars

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

August 25, 1996

Volume

172

Issue

1

Start / End Page

52 / 59

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes, Cytotoxic
  • Melanoma
  • Immunology
  • Humans
  • HLA-A2 Antigen
  • Clone Cells
  • Cell Division
  • CD8 Antigens
  • CD4 Antigens
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Darrow, T. L., Abdel-Wahab, Z., Quinn-Allen, M. A., & Seigler, H. F. (1996). Recognition and lysis of human melanoma by a CD3+, CD4+, CD8- T-cell clone restricted by HLA-A2. Cell Immunol, 172(1), 52–59. https://doi.org/10.1006/cimm.1996.0214
Darrow, T. L., Z. Abdel-Wahab, M. A. Quinn-Allen, and H. F. Seigler. “Recognition and lysis of human melanoma by a CD3+, CD4+, CD8- T-cell clone restricted by HLA-A2.Cell Immunol 172, no. 1 (August 25, 1996): 52–59. https://doi.org/10.1006/cimm.1996.0214.
Darrow TL, Abdel-Wahab Z, Quinn-Allen MA, Seigler HF. Recognition and lysis of human melanoma by a CD3+, CD4+, CD8- T-cell clone restricted by HLA-A2. Cell Immunol. 1996 Aug 25;172(1):52–9.
Darrow, T. L., et al. “Recognition and lysis of human melanoma by a CD3+, CD4+, CD8- T-cell clone restricted by HLA-A2.Cell Immunol, vol. 172, no. 1, Aug. 1996, pp. 52–59. Pubmed, doi:10.1006/cimm.1996.0214.
Darrow TL, Abdel-Wahab Z, Quinn-Allen MA, Seigler HF. Recognition and lysis of human melanoma by a CD3+, CD4+, CD8- T-cell clone restricted by HLA-A2. Cell Immunol. 1996 Aug 25;172(1):52–59.
Journal cover image

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

August 25, 1996

Volume

172

Issue

1

Start / End Page

52 / 59

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes, Cytotoxic
  • Melanoma
  • Immunology
  • Humans
  • HLA-A2 Antigen
  • Clone Cells
  • Cell Division
  • CD8 Antigens
  • CD4 Antigens