Multiple assay characterization of murine monoclonal antimelanoma antibodies.

Journal Article (Journal Article)

Selected murine monoclonal antimelanoma antibodies have been extensively evaluated using multiple radioimmunoassay methods, immunoprecipitation and immunohistochemistry. Use of this approach has permitted more complete definition of the specificity of these reagents and provided information regarding the nature and distribution of the respective tumor associated antigens (TAA). Several patterns of reactivity were identified. Some of the reagents were highly reactive with melanomas but also with a variety of tissues of nonmelanoma origin. Others were less highly reactive but of greater specificity for melanoma. Finally, certain of the reagents were poorly reactive in the assays utilized or demonstrated assay-dependent reactivity. None of the included monoclonal antibodies appeared to detect TAA restricted in distribution solely to melanoma.

Full Text

Duke Authors

Cited Authors

  • Stuhlmiller, GM; Borowitz, MJ; Croker, BP; Seigler, HF

Published Date

  • 1982

Published In

Volume / Issue

  • 1 / 4

Start / End Page

  • 447 - 460

PubMed ID

  • 6208139

International Standard Serial Number (ISSN)

  • 0272-457X

Digital Object Identifier (DOI)

  • 10.1089/hyb.1.1982.1.447


  • eng

Conference Location

  • United States