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Inhibition of the growth of human melanoma metastases in nude mice by melanoma-specific murine monoclonal antibody.

Publication ,  Journal Article
Abdel-Wahab, ZA; Darrow, TL; Vervaert, CE; Giannopoulou, AA; Li, W; Seigler, HF
Published in: Surg Oncol
April 1992

The administration of anti-melanoma murine monoclonal antibody (MAB) 16.C8 (IgG2a) to nude mice bearing established human melanoma lung or liver metastases resulted in a significant inhibition of tumour growth. A total dose of 2 mg of affinity purified 16.C8 caused complete inhibition of tumour growth in 89 and 100% of animals in the liver and lung model, respectively. In contrast, a significant tumour growth was found in most control animals which received an irrelevant IgG2a MAB or 2% human serum albumin in Hanks Balanced Salt Solution (HBSS). The MAB was most effective when treatment was started on day 1 or 4 following tumour inoculation. When the 16.C8 MAB treatment was delayed 7 or 14 days, 33 and 67% of 16.C8 treated animals, respectively, developed tumours. The MAB-mediated anti-tumour activity appeared to be dose dependent, and the effect of a suboptimal dose was potentiated by the concomitant administration of recombinant interleukin 2 (rIL-2). Recombinant IL-2 alone in a similar dose did not elicit comparable anti-tumour activity. Moreover, the MAB 16.C8 inhibited tumour growth in irradiated animals which may suggest the involvement of host-radioresistant cellular elements in the 16.C8 antibody-mediated anti-tumour activities in nude mice. These results suggest that MAB 16.C8 alone or combined with rIL-2 may prove useful in the immunotherapy of metastatic melanoma.

Duke Scholars

Published In

Surg Oncol

DOI

ISSN

0960-7404

Publication Date

April 1992

Volume

1

Issue

2

Start / End Page

115 / 125

Location

Netherlands

Related Subject Headings

  • Whole-Body Irradiation
  • Tumor Cells, Cultured
  • Skin Neoplasms
  • Recombinant Proteins
  • Oncology & Carcinogenesis
  • Neoplasm Transplantation
  • Mice, Nude
  • Mice, Inbred BALB C
  • Mice
  • Melanoma, Experimental
 

Citation

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ICMJE
MLA
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Abdel-Wahab, Z. A., Darrow, T. L., Vervaert, C. E., Giannopoulou, A. A., Li, W., & Seigler, H. F. (1992). Inhibition of the growth of human melanoma metastases in nude mice by melanoma-specific murine monoclonal antibody. Surg Oncol, 1(2), 115–125. https://doi.org/10.1016/0960-7404(92)90024-f
Abdel-Wahab, Z. A., T. L. Darrow, C. E. Vervaert, A. A. Giannopoulou, W. Li, and H. F. Seigler. “Inhibition of the growth of human melanoma metastases in nude mice by melanoma-specific murine monoclonal antibody.Surg Oncol 1, no. 2 (April 1992): 115–25. https://doi.org/10.1016/0960-7404(92)90024-f.
Abdel-Wahab ZA, Darrow TL, Vervaert CE, Giannopoulou AA, Li W, Seigler HF. Inhibition of the growth of human melanoma metastases in nude mice by melanoma-specific murine monoclonal antibody. Surg Oncol. 1992 Apr;1(2):115–25.
Abdel-Wahab, Z. A., et al. “Inhibition of the growth of human melanoma metastases in nude mice by melanoma-specific murine monoclonal antibody.Surg Oncol, vol. 1, no. 2, Apr. 1992, pp. 115–25. Pubmed, doi:10.1016/0960-7404(92)90024-f.
Abdel-Wahab ZA, Darrow TL, Vervaert CE, Giannopoulou AA, Li W, Seigler HF. Inhibition of the growth of human melanoma metastases in nude mice by melanoma-specific murine monoclonal antibody. Surg Oncol. 1992 Apr;1(2):115–125.
Journal cover image

Published In

Surg Oncol

DOI

ISSN

0960-7404

Publication Date

April 1992

Volume

1

Issue

2

Start / End Page

115 / 125

Location

Netherlands

Related Subject Headings

  • Whole-Body Irradiation
  • Tumor Cells, Cultured
  • Skin Neoplasms
  • Recombinant Proteins
  • Oncology & Carcinogenesis
  • Neoplasm Transplantation
  • Mice, Nude
  • Mice, Inbred BALB C
  • Mice
  • Melanoma, Experimental