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An experimental model of chronic myocardial hibernation.

Publication ,  Journal Article
St Louis, JD; Hughes, GC; Kypson, AP; DeGrado, TR; Donovan, CL; Coleman, RE; Yin, B; Steenbergen, C; Landolfo, KP; Lowe, JE
Published in: Ann Thorac Surg
May 2000

BACKGROUND: Hibernating myocardium describes persistently impaired ventricular function at rest caused by reduced coronary blood flow. However, a realistic animal model reproducing this chronic ischemic state does not exist. The purpose of this study was to explore whether chronic low-flow hibernation could be produced in swine. METHODS: Miniswine underwent 90% stenosis of the left circumflex coronary artery. Positron emission tomography and dobutamine stress echocardiography were performed 3 and 30 days (n = 6) or 14 days (n = 4) after occlusion to evaluate myocardial blood flow and viability. Triphenyl tetrazolium chloride assessed percent infarction. Electron microscopy was used to identify cellular changes characteristic of hibernating myocardium. RESULTS: Positron emission tomography (13N-labeled-ammonia) 3 days after occlusion demonstrated a significant reduction in myocardial blood flow in the left circumflex distribution. This reduced flow was accompanied by increased glucose use (18F-fluorodeoxyglucose), which is consistent with hibernating myocardium. Thirty days after occlusion, positron emission tomography demonstrated persistent low flow with increased glucose use in the left circumflex distribution. Dobutamine stress echocardiography 3 days after occlusion demonstrated severe hypocontractility at rest in the left circumflex region. Regional wall motion improved with low-dose dobutamine followed by deterioration at higher doses (biphasic response), findings consistent with hibernating myocardium. The results of dobutamine stress echocardiography were unchanged 30 days after occlusion. Triphenyl tetrazolium chloride staining (n = 6) revealed a mean of 8% +/- 2% infarction of the area-at-risk localized to the endocardial surface. Electron microscopy (n = 4) 14 days after occlusion demonstrated loss of contractile elements and large areas of glycogen accumulation within viable cardiomyocytes, also characteristic of hibernating myocardium. CONCLUSIONS: Chronic low-flow myocardial hibernation can be reproduced in an animal model after partial coronary occlusion. This model may prove useful in the study of the mechanisms underlying hibernating myocardium and the use of therapies designed to improve blood flow to the heart.

Duke Scholars

Published In

Ann Thorac Surg

DOI

ISSN

0003-4975

Publication Date

May 2000

Volume

69

Issue

5

Start / End Page

1351 / 1357

Location

Netherlands

Related Subject Headings

  • Tomography, Emission-Computed
  • Tissue Survival
  • Swine, Miniature
  • Swine
  • Respiratory System
  • Myocardium
  • Myocardial Stunning
  • Male
  • Echocardiography
  • Dobutamine
 

Citation

APA
Chicago
ICMJE
MLA
NLM
St Louis, J. D., Hughes, G. C., Kypson, A. P., DeGrado, T. R., Donovan, C. L., Coleman, R. E., … Lowe, J. E. (2000). An experimental model of chronic myocardial hibernation. Ann Thorac Surg, 69(5), 1351–1357. https://doi.org/10.1016/s0003-4975(00)01130-9
St Louis, J. D., G. C. Hughes, A. P. Kypson, T. R. DeGrado, C. L. Donovan, R. E. Coleman, B. Yin, C. Steenbergen, K. P. Landolfo, and J. E. Lowe. “An experimental model of chronic myocardial hibernation.Ann Thorac Surg 69, no. 5 (May 2000): 1351–57. https://doi.org/10.1016/s0003-4975(00)01130-9.
St Louis JD, Hughes GC, Kypson AP, DeGrado TR, Donovan CL, Coleman RE, et al. An experimental model of chronic myocardial hibernation. Ann Thorac Surg. 2000 May;69(5):1351–7.
St Louis, J. D., et al. “An experimental model of chronic myocardial hibernation.Ann Thorac Surg, vol. 69, no. 5, May 2000, pp. 1351–57. Pubmed, doi:10.1016/s0003-4975(00)01130-9.
St Louis JD, Hughes GC, Kypson AP, DeGrado TR, Donovan CL, Coleman RE, Yin B, Steenbergen C, Landolfo KP, Lowe JE. An experimental model of chronic myocardial hibernation. Ann Thorac Surg. 2000 May;69(5):1351–1357.
Journal cover image

Published In

Ann Thorac Surg

DOI

ISSN

0003-4975

Publication Date

May 2000

Volume

69

Issue

5

Start / End Page

1351 / 1357

Location

Netherlands

Related Subject Headings

  • Tomography, Emission-Computed
  • Tissue Survival
  • Swine, Miniature
  • Swine
  • Respiratory System
  • Myocardium
  • Myocardial Stunning
  • Male
  • Echocardiography
  • Dobutamine