Correlation of UVC and UVB cytotoxicity with the induction of specific photoproducts in T-lymphocytes and fibroblasts from normal human donors.

Published

Journal Article

By using specific monoclonal antibodies in situ and a computer-assisted image analysis system we have determined the relative induction of cyclobutane dimers, (6-4) photoproducts and Dewar isomers in human mononuclear cells and fibroblasts following irradiation with UVC, broad-spectrum UVB and narrow-spectrum UVB. The lamps produced these lesions in different proportions, with broad-spectrum UVB inducing a greater combined yield of (6-4) photoproducts and Dewar isomers per cyclobutane dimer than UVC or narrow-spectrum UVB. The relative induction ratios of (6-4) photoproducts compared to cyclobutane dimers were 0.15, 0.21 and 0.10 following irradiation with UVC, broad- or narrow-spectrum UVB, respectively. Although Dewar isomers were induced by UVC, their relative rate of formation compared to cyclobutane dimers was significantly greater after irradiation with either broad-spectrum or narrow-spectrum UVB. These values were 0.001, 0.07 and 0.07, respectively. With each lamp source, we have determined the survival of normal human T-lymphocytes and fibroblasts at fluences, which induce equivalent yields of cyclobutane dimers, (6-4) photoproducts or (6-4) photoproducts plus Dewar isomers. Killing of fibroblasts appears to be associated with (6-4) photoproduct formation, whereas killing of T-lymphocytes seems to be mediated by combined (6-4) plus Dewar yields. These results emphasize the need to study the biological effects of UVB because cellular responses may be different from those following UVC irradiation.

Full Text

Cited Authors

  • Clingen, PH; Arlett, CF; Cole, J; Waugh, AP; Lowe, JE; Harcourt, SA; Hermanova, N; Roza, L; Mori, T; Nikaido, O

Published Date

  • February 1995

Published In

Volume / Issue

  • 61 / 2

Start / End Page

  • 163 - 170

PubMed ID

  • 7899505

Pubmed Central ID

  • 7899505

Electronic International Standard Serial Number (EISSN)

  • 1751-1097

International Standard Serial Number (ISSN)

  • 0031-8655

Digital Object Identifier (DOI)

  • 10.1111/j.1751-1097.1995.tb03955.x

Language

  • eng