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Lack of evidence for MHC-unrestricted (atypical) recognition of mucin by mucinous pancreatic tumour-reactive T-cells.

Publication ,  Journal Article
Selvan, RS; Pappas, TN; Ward, FE
Published in: Br J Cancer
February 2000

Cytotoxic T-cells generated against heterologous, mucinous pancreatic tumour cells were shown to recognize mucin in a major histocombatibility complex (MHC)-unrestricted fashion. In contrast, the present study demonstrates a typical allogeneic response of heterologous cytotoxic T-cells established against mucin-expressing pancreatic tumour cells. Heterologous cytotoxic T cells lysed targets that were used as stimulators and other targets that shared human leucocyte antigen (HLA) with the stimulator. These cytotoxic T-cells lysed mucin-expressing stimulator cells but not autologous tumour cells in spite of expressing mucin on their surface. Likewise, tumour-infiltrating CD4+ T-cells proliferated against its own tumour cell target, while such T-cells did not respond to heterologous, mucin-expressing pancreatic tumour cells. Culturing heterologous tumour-specific cytotoxic T-cells with purified pancreatic tumour cell-mucin rendered them unresponsive to their target cells. Furthermore, purified mucin did not produce a mucin-specific response in mucinous pancreatic tumour patients' primary T-cells even in the presence of antigen-presenting cells. Our study finds no evidence for MHC-unrestricted recognition of mucin by pancreatic cancer patients' T-cells.

Duke Scholars

Published In

Br J Cancer

DOI

ISSN

0007-0920

Publication Date

February 2000

Volume

82

Issue

3

Start / End Page

691 / 701

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes, Cytotoxic
  • Pancreatic Neoplasms
  • Oncology & Carcinogenesis
  • Mucins
  • Major Histocompatibility Complex
  • Killer Cells, Lymphokine-Activated
  • Immunohistochemistry
  • Humans
  • Flow Cytometry
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Selvan, R. S., Pappas, T. N., & Ward, F. E. (2000). Lack of evidence for MHC-unrestricted (atypical) recognition of mucin by mucinous pancreatic tumour-reactive T-cells. Br J Cancer, 82(3), 691–701. https://doi.org/10.1054/bjoc.1999.0982
Selvan, R. S., T. N. Pappas, and F. E. Ward. “Lack of evidence for MHC-unrestricted (atypical) recognition of mucin by mucinous pancreatic tumour-reactive T-cells.Br J Cancer 82, no. 3 (February 2000): 691–701. https://doi.org/10.1054/bjoc.1999.0982.
Selvan, R. S., et al. “Lack of evidence for MHC-unrestricted (atypical) recognition of mucin by mucinous pancreatic tumour-reactive T-cells.Br J Cancer, vol. 82, no. 3, Feb. 2000, pp. 691–701. Pubmed, doi:10.1054/bjoc.1999.0982.

Published In

Br J Cancer

DOI

ISSN

0007-0920

Publication Date

February 2000

Volume

82

Issue

3

Start / End Page

691 / 701

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes, Cytotoxic
  • Pancreatic Neoplasms
  • Oncology & Carcinogenesis
  • Mucins
  • Major Histocompatibility Complex
  • Killer Cells, Lymphokine-Activated
  • Immunohistochemistry
  • Humans
  • Flow Cytometry