Short-chain fatty acids stimulate colonic transit via intraluminal 5-HT release in rats.

Published

Journal Article

We studied whether physiological concentration of short-chain fatty acids (SCFAs) affects colonic transit and colonic motility in conscious rats. Intraluminal administration of SCFAs (100-200 mM) into the proximal colon significantly accelerated colonic transit. The stimulatory effect of SCFAs on colonic transit was abolished by perivagal capsaicin treatment, atropine, hexamethonium, and vagotomy, but not by guanethidine. The stimulatory effect of SCFAs on colonic transit was also abolished by intraluminal pretreatment with lidocaine and a 5-hydroxytryptamine (HT)(3) receptor antagonist. Intraluminal administration of SCFAs provoked contractions at the proximal colon, which migrated to the mid- and distal colon. SCFAs caused a significant increase in the luminal concentration of 5-HT of the vascularly isolated and luminally perfused rat colon ex vivo. It is suggested that the release of 5-HT from enterochromaffin cells in response to SCFAs stimulates 5-HT(3) receptors located on the vagal sensory fibers. The sensory information is transferred to the vagal efferent and stimulates the release of acetylcholine from the colonic myenteric plexus, resulting in muscle contraction.

Full Text

Duke Authors

Cited Authors

  • Fukumoto, S; Tatewaki, M; Yamada, T; Fujimiya, M; Mantyh, C; Voss, M; Eubanks, S; Harris, M; Pappas, TN; Takahashi, T

Published Date

  • May 2003

Published In

Volume / Issue

  • 284 / 5

Start / End Page

  • R1269 - R1276

PubMed ID

  • 12676748

Pubmed Central ID

  • 12676748

International Standard Serial Number (ISSN)

  • 0363-6119

Digital Object Identifier (DOI)

  • 10.1152/ajpregu.00442.2002

Language

  • eng

Conference Location

  • United States