Loss of p53 in benzene-induced thymic lymphomas in p53+/- mice: evidence of chromosomal recombination.


Journal Article

The purpose of this study was to examine the role of chromosomal recombination in mediating p53 loss in benzene-induced thymic lymphomas in C57BL/6-Trp53 haploinsufficient (N5) mice (p53+/- mice). We characterized loss of heterozygosity (LOH) on chromosome 11 using seven microsatellite markers in 27 benzene-induced and 6 spontaneous thymic lymphomas. Eleven patterns of LOH were found between the induced and spontaneous tumors, with only one pattern being in common between the tumor groups. Nearly 90% (24 of 27) of benzene-induced tumors exhibited loss of the functional p53 allele locus, and 83% (20 of 24) of these tumors retained two copies of the disrupted p53 allele. The results indicate that benzene induces a high frequency of LOH on chromosome 11 in p53+/- mice, likely mediated by aberrant chromosomal recombination.

Full Text

Cited Authors

  • Boley, SE; Anderson, EE; French, JE; Donehower, LA; Walker, DB; Recio, L

Published Date

  • June 2000

Published In

Volume / Issue

  • 60 / 11

Start / End Page

  • 2831 - 2835

PubMed ID

  • 10850423

Pubmed Central ID

  • 10850423

Electronic International Standard Serial Number (EISSN)

  • 1538-7445

International Standard Serial Number (ISSN)

  • 0008-5472


  • eng