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Unique chromosomal rearrangement and mucin production in a novel salivary myoepithelial cell strain.

Publication ,  Journal Article
Witsell, DL; Weissler, MC; Livanos, E; Bova-Hill, C; Gilmer, TM
Published in: Arch Otolaryngol Head Neck Surg
October 1993

Pleomorphic adenoma is the most frequently occurring benign neoplasm of the salivary glands. We describe the culture characteristics, biochemical properties, immunohistochemical staining, and genetic rearrangements found in a novel cell strain (UNC4) established from a human benign pleomorphic adenoma. Serum and/or butyrate stimulation of UNC4 cultures results in upregulation of mucin production. This is confirmed by periodic acid-Schiff, periodic acid-Schiff digest, alcian blue, and mucicarmine staining as well as by gel electrophoresis. Fluorescent immunohistochemical studies detect cellular cytokeratin, desmin, and epithelial membrane antigen. Immunofluorescent staining for S100 protein is negative. Examination of the karyotype of UNC4 reveals a unique rearrangement between one chromosome 8 and the two chromosome 9s involving the 8q12 locus. UNC4 represents an in vitro model of a benign salivary gland neoplasm that can provide the basis for further molecular and biochemical studies on genetic rearrangement and salivary mucin production.

Duke Scholars

Published In

Arch Otolaryngol Head Neck Surg

DOI

ISSN

0886-4470

Publication Date

October 1993

Volume

119

Issue

10

Start / End Page

1151 / 1157

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Salivary Glands
  • Salivary Gland Neoplasms
  • Otorhinolaryngology
  • Mucins
  • Middle Aged
  • Karyotyping
  • Immunohistochemistry
  • Humans
  • Female
 

Citation

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Witsell, D. L., Weissler, M. C., Livanos, E., Bova-Hill, C., & Gilmer, T. M. (1993). Unique chromosomal rearrangement and mucin production in a novel salivary myoepithelial cell strain. Arch Otolaryngol Head Neck Surg, 119(10), 1151–1157. https://doi.org/10.1001/archotol.1993.01880220107013
Witsell, D. L., M. C. Weissler, E. Livanos, C. Bova-Hill, and T. M. Gilmer. “Unique chromosomal rearrangement and mucin production in a novel salivary myoepithelial cell strain.Arch Otolaryngol Head Neck Surg 119, no. 10 (October 1993): 1151–57. https://doi.org/10.1001/archotol.1993.01880220107013.
Witsell DL, Weissler MC, Livanos E, Bova-Hill C, Gilmer TM. Unique chromosomal rearrangement and mucin production in a novel salivary myoepithelial cell strain. Arch Otolaryngol Head Neck Surg. 1993 Oct;119(10):1151–7.
Witsell, D. L., et al. “Unique chromosomal rearrangement and mucin production in a novel salivary myoepithelial cell strain.Arch Otolaryngol Head Neck Surg, vol. 119, no. 10, Oct. 1993, pp. 1151–57. Pubmed, doi:10.1001/archotol.1993.01880220107013.
Witsell DL, Weissler MC, Livanos E, Bova-Hill C, Gilmer TM. Unique chromosomal rearrangement and mucin production in a novel salivary myoepithelial cell strain. Arch Otolaryngol Head Neck Surg. 1993 Oct;119(10):1151–1157.

Published In

Arch Otolaryngol Head Neck Surg

DOI

ISSN

0886-4470

Publication Date

October 1993

Volume

119

Issue

10

Start / End Page

1151 / 1157

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Salivary Glands
  • Salivary Gland Neoplasms
  • Otorhinolaryngology
  • Mucins
  • Middle Aged
  • Karyotyping
  • Immunohistochemistry
  • Humans
  • Female