Regulation of lipoprotein lipase activity and mRNA in the mammary gland of the lactating mouse.
Journal Article (Journal Article)
We examined the effects of reproductive stage and fasting on lipoprotein lipase (LPL) activity and mRNA in the mouse mammary gland. Heparin-releasable and cell-associated LPL activity rose immediately after birth, followed 1-2 days later by an increase in LPL mRNA. Fasting decreased LPL activity in the mammary gland at all reproductive stages. During lactation, both milk and heparin-releasable LPL were substantially decreased by an overnight fast, whereas cell-associated LPL was less affected and LPL mRNA did not change. These studies indicate that the extracellular, heparin-releasable, fraction of mammary LPL activity responds most rapidly to alterations in physiological state, usually accompanied by smaller changes in cellular enzyme activity. Changes in the level of LPL mRNA were seen only during the transition from pregnancy to lactation, and these tended to follow, rather than precede, changes in enzyme activity. We conclude that in the mammary gland as in adipose tissue, LPL is regulated primarily at the translational and post-translational level.
Full Text
Duke Authors
Cited Authors
- Jensen, DR; Gavigan, S; Sawicki, V; Witsell, DL; Eckel, RH; Neville, MC
Published Date
- March 1, 1994
Published In
Volume / Issue
- 298 ( Pt 2) /
Start / End Page
- 321 - 327
PubMed ID
- 8135737
Pubmed Central ID
- PMC1137942
International Standard Serial Number (ISSN)
- 0264-6021
Digital Object Identifier (DOI)
- 10.1042/bj2980321
Language
- eng
Conference Location
- England