Endotoxin and NO induce MIP-1gamma gene transcription in ANA-1 murine macrophages.
Publication
, Journal Article
Guo, HT; Cai, CQ; Kuo, PC
Published in: Biochem Biophys Res Commun
November 2, 2000
The host response to gram-negative endotoxin is characterized by an influx of inflammatory cells into host tissues, mediated in part by localized production of chemokines. In this study, using subtractive suppression hybridization analysis, we demonstrate that ANA-1 murine macrophages produce the CC chemokine, MIP-1gamma, in response to LPS-mediated NO production. Gene transcription and protein translation are upregulated in the setting of LPS-induced NO synthesis. However, NO alone is a necessary but insufficient cofactor for induction of MIP-1gamma protein expression; an NO-independent LPS signalling pathway is also required. This study suggests a novel mechanism by which NO modulates the host inflammatory response to endotoxin.
Duke Scholars
Published In
Biochem Biophys Res Commun
DOI
ISSN
0006-291X
Publication Date
November 2, 2000
Volume
277
Issue
3
Start / End Page
617 / 621
Location
United States
Related Subject Headings
- Transcription, Genetic
- Nitric Oxide
- Mice
- Macrophages
- Macrophage Inflammatory Proteins
- Lipopolysaccharides
- Inflammation
- Gene Expression Regulation
- Dose-Response Relationship, Drug
- Chemokine CCL4
Citation
APA
Chicago
ICMJE
MLA
NLM
Guo, H. T., Cai, C. Q., & Kuo, P. C. (2000). Endotoxin and NO induce MIP-1gamma gene transcription in ANA-1 murine macrophages. Biochem Biophys Res Commun, 277(3), 617–621. https://doi.org/10.1006/bbrc.2000.3718
Guo, H. T., C. Q. Cai, and P. C. Kuo. “Endotoxin and NO induce MIP-1gamma gene transcription in ANA-1 murine macrophages.” Biochem Biophys Res Commun 277, no. 3 (November 2, 2000): 617–21. https://doi.org/10.1006/bbrc.2000.3718.
Guo HT, Cai CQ, Kuo PC. Endotoxin and NO induce MIP-1gamma gene transcription in ANA-1 murine macrophages. Biochem Biophys Res Commun. 2000 Nov 2;277(3):617–21.
Guo, H. T., et al. “Endotoxin and NO induce MIP-1gamma gene transcription in ANA-1 murine macrophages.” Biochem Biophys Res Commun, vol. 277, no. 3, Nov. 2000, pp. 617–21. Pubmed, doi:10.1006/bbrc.2000.3718.
Guo HT, Cai CQ, Kuo PC. Endotoxin and NO induce MIP-1gamma gene transcription in ANA-1 murine macrophages. Biochem Biophys Res Commun. 2000 Nov 2;277(3):617–621.
Published In
Biochem Biophys Res Commun
DOI
ISSN
0006-291X
Publication Date
November 2, 2000
Volume
277
Issue
3
Start / End Page
617 / 621
Location
United States
Related Subject Headings
- Transcription, Genetic
- Nitric Oxide
- Mice
- Macrophages
- Macrophage Inflammatory Proteins
- Lipopolysaccharides
- Inflammation
- Gene Expression Regulation
- Dose-Response Relationship, Drug
- Chemokine CCL4