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A transcriptional repressor of osteopontin expression in the 4T1 murine breast cancer cell line.

Publication ,  Journal Article
Gao, C; Mi, Z; Guo, H; Wei, J; Wai, PY; Kuo, PC
Published in: Biochem Biophys Res Commun
September 3, 2004

Osteopontin (OPN) is a highly hydrophilic and negatively charged sialoprotein of approximately 298 amino acids which is an important mediator of tumor metastatic behavior. We have previously demonstrated that endotoxin-dependent OPN gene transcription is regulated by a constitutive transcriptional repressor protein, heterogeneous nuclear ribonucleoprotein A/B (hnRNP-A/B). However, in the context of cancer, the role of hnRNP-A/B in the transcriptional regulation of OPN and its metastasis-promoting functions has not been previously studied. We examined hnRNP-A/B in the 4T1 murine mammary epithelial tumor cell line, a thioguanine resistant subline which closely mimics stage IV breast cancer in humans. Our data indicate that hnRNP-A/B p37 binds to the OPN promoter, significantly decreases OPN promoter activity and mRNA levels, ablates OPN protein expression, and inhibits OPN dependent in vitro correlates of metastatic behavior, motility, and invasion. These results are unique and may suggest new therapies to re-establish loco-regional control of cancers.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

September 3, 2004

Volume

321

Issue

4

Start / End Page

1010 / 1016

Location

United States

Related Subject Headings

  • Transfection
  • Sialoglycoproteins
  • Repressor Proteins
  • RNA, Neoplasm
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Osteopontin
  • Neoplasm Proteins
  • Neoplasm Invasiveness
  • Mice
 

Citation

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ICMJE
MLA
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Gao, C., Mi, Z., Guo, H., Wei, J., Wai, P. Y., & Kuo, P. C. (2004). A transcriptional repressor of osteopontin expression in the 4T1 murine breast cancer cell line. Biochem Biophys Res Commun, 321(4), 1010–1016. https://doi.org/10.1016/j.bbrc.2004.07.063
Gao, Chengjiang, Zhiyong Mi, Hongtao Guo, Junping Wei, Philip Y. Wai, and Paul C. Kuo. “A transcriptional repressor of osteopontin expression in the 4T1 murine breast cancer cell line.Biochem Biophys Res Commun 321, no. 4 (September 3, 2004): 1010–16. https://doi.org/10.1016/j.bbrc.2004.07.063.
Gao C, Mi Z, Guo H, Wei J, Wai PY, Kuo PC. A transcriptional repressor of osteopontin expression in the 4T1 murine breast cancer cell line. Biochem Biophys Res Commun. 2004 Sep 3;321(4):1010–6.
Gao, Chengjiang, et al. “A transcriptional repressor of osteopontin expression in the 4T1 murine breast cancer cell line.Biochem Biophys Res Commun, vol. 321, no. 4, Sept. 2004, pp. 1010–16. Pubmed, doi:10.1016/j.bbrc.2004.07.063.
Gao C, Mi Z, Guo H, Wei J, Wai PY, Kuo PC. A transcriptional repressor of osteopontin expression in the 4T1 murine breast cancer cell line. Biochem Biophys Res Commun. 2004 Sep 3;321(4):1010–1016.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

September 3, 2004

Volume

321

Issue

4

Start / End Page

1010 / 1016

Location

United States

Related Subject Headings

  • Transfection
  • Sialoglycoproteins
  • Repressor Proteins
  • RNA, Neoplasm
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Osteopontin
  • Neoplasm Proteins
  • Neoplasm Invasiveness
  • Mice