Skip to main content
Journal cover image

Role of plasminogen activator and of 92-KDa type IV collagenase in glioblastoma invasion using an in vitro matrigel model.

Publication ,  Journal Article
Rao, JS; Steck, PA; Tofilon, P; Boyd, D; Ali-Osman, F; Stetler-Stevenson, WG; Liotta, LA; Sawaya, R
Published in: J Neurooncol
1994

The invasive nature of human gliomas represents a major factor in preventing their total resection. The exact nature of the underlying mechanisms of tumor cell invasion are still unclear. In this study, we have quantitatively assayed a glioblastoma cell line for its ability to migrate through a polycarbonate filter coated with matrigel which contains a complex of multiple basement membrane components. At 48 h the glioblastoma cell line (U251) showed a rate of invasiveness of 42% and also dependent on the concentration of matrigel. The U251 cell line produced a urokinase type plasminogen activator and a 92-KDa type IV collagenase. Both enzymes were inhibited by the addition of uPA and 92-KDa type IV collagenase antibodies. Those same antibodies reduced the invasion rate of U251 cells from 42% to 12 and 21%, respectively. Similarly, the addition of epsilon-aminocaproic acid (a plasmin inhibitor) or tissue inhibitor of metalloprotease (TIMP2, a collagenase inhibitor) reduced the invasiveness of U251 cells from 42% to 14% and 10%, respectively. Additionally, the other two glioblastoma cell lines (LG11, UWR1) and astrocytes showed a rate of invasiveness at 41%, 61% and 12%, respectively. Finally, the addition of hyaluronic acid to the matrigel, a constituent of brain extracellular matrix, enhanced the rate of invasion. These findings provide evidence for the role of serine proteases and metalloproteases in facilitating the invasion of extracellular matrix components by glioblastoma cell line and suggest a therapeutic role for protease inhibitors in attempting to minimize the invasive propensity of gliomas.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Neurooncol

DOI

ISSN

0167-594X

Publication Date

1994

Volume

18

Issue

2

Start / End Page

129 / 138

Location

United States

Related Subject Headings

  • Urokinase-Type Plasminogen Activator
  • Tumor Cells, Cultured
  • Proteoglycans
  • Plasminogen Activators
  • Oncology & Carcinogenesis
  • Neoplasm Invasiveness
  • Molecular Weight
  • Models, Biological
  • Laminin
  • Hyaluronic Acid
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Rao, J. S., Steck, P. A., Tofilon, P., Boyd, D., Ali-Osman, F., Stetler-Stevenson, W. G., … Sawaya, R. (1994). Role of plasminogen activator and of 92-KDa type IV collagenase in glioblastoma invasion using an in vitro matrigel model. J Neurooncol, 18(2), 129–138. https://doi.org/10.1007/BF01050419
Rao, J. S., P. A. Steck, P. Tofilon, D. Boyd, F. Ali-Osman, W. G. Stetler-Stevenson, L. A. Liotta, and R. Sawaya. “Role of plasminogen activator and of 92-KDa type IV collagenase in glioblastoma invasion using an in vitro matrigel model.J Neurooncol 18, no. 2 (1994): 129–38. https://doi.org/10.1007/BF01050419.
Rao JS, Steck PA, Tofilon P, Boyd D, Ali-Osman F, Stetler-Stevenson WG, et al. Role of plasminogen activator and of 92-KDa type IV collagenase in glioblastoma invasion using an in vitro matrigel model. J Neurooncol. 1994;18(2):129–38.
Rao, J. S., et al. “Role of plasminogen activator and of 92-KDa type IV collagenase in glioblastoma invasion using an in vitro matrigel model.J Neurooncol, vol. 18, no. 2, 1994, pp. 129–38. Pubmed, doi:10.1007/BF01050419.
Rao JS, Steck PA, Tofilon P, Boyd D, Ali-Osman F, Stetler-Stevenson WG, Liotta LA, Sawaya R. Role of plasminogen activator and of 92-KDa type IV collagenase in glioblastoma invasion using an in vitro matrigel model. J Neurooncol. 1994;18(2):129–138.
Journal cover image

Published In

J Neurooncol

DOI

ISSN

0167-594X

Publication Date

1994

Volume

18

Issue

2

Start / End Page

129 / 138

Location

United States

Related Subject Headings

  • Urokinase-Type Plasminogen Activator
  • Tumor Cells, Cultured
  • Proteoglycans
  • Plasminogen Activators
  • Oncology & Carcinogenesis
  • Neoplasm Invasiveness
  • Molecular Weight
  • Models, Biological
  • Laminin
  • Hyaluronic Acid