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Deletions and rearrangements inactivate the p16INK4 gene in human glioma cells.

Publication ,  Journal Article
Srivenugopal, KS; Ali-Osman, F
Published in: Oncogene
May 2, 1996

Structural alterations in the p16INK4 gene were examined in early passage human glioma cell lines and related to the expression of p16 transcripts and protein. Using the Southern blot approach, we observed both homozygous and hemizygous deletions, as well as rearrangements of the p16 and p15 genes in 5 of the 7 cell lines (71%). Two cell lines, MGR3 and HBT28, revealed hemizygous deletion of the p16 and p15 genes combined with indistinguishable rearrangements of the remaining p15-p16 locus that resulted in loss of exon 2 sequences for p15 and p16, but retention of p16 exon 1; neither of these cell lines expressed p16 mRNA. Data for a third cell line, MGR2, indicated a similar, but unique rearrangement involving the p15 and p16 genes. MGR2, which retained a single wild-type p15-p16 locus, showed expression of p16 transcript, but not of p16 protein as indicated by Western blot analysis. All the glioma cell lines expressed similar levels of the retinoblastoma protein and no amplification of the cyclin-dependent kinase 4 gene. These results demonstrate that human glioma cells contain p16 gene microdeletions and rearrangements that contribute to inactivation of the cell cycle regulatory protein.

Duke Scholars

Published In

Oncogene

ISSN

0950-9232

Publication Date

May 2, 1996

Volume

12

Issue

9

Start / End Page

2029 / 2034

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • RNA, Messenger
  • Oncology & Carcinogenesis
  • Humans
  • Glioma
  • Gene Rearrangement
  • Gene Deletion
  • Cyclin-Dependent Kinase Inhibitor p16
  • Carrier Proteins
  • Brain Neoplasms
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Srivenugopal, K. S., & Ali-Osman, F. (1996). Deletions and rearrangements inactivate the p16INK4 gene in human glioma cells. Oncogene, 12(9), 2029–2034.
Srivenugopal, K. S., and F. Ali-Osman. “Deletions and rearrangements inactivate the p16INK4 gene in human glioma cells.Oncogene 12, no. 9 (May 2, 1996): 2029–34.
Srivenugopal KS, Ali-Osman F. Deletions and rearrangements inactivate the p16INK4 gene in human glioma cells. Oncogene. 1996 May 2;12(9):2029–34.
Srivenugopal, K. S., and F. Ali-Osman. “Deletions and rearrangements inactivate the p16INK4 gene in human glioma cells.Oncogene, vol. 12, no. 9, May 1996, pp. 2029–34.
Srivenugopal KS, Ali-Osman F. Deletions and rearrangements inactivate the p16INK4 gene in human glioma cells. Oncogene. 1996 May 2;12(9):2029–2034.

Published In

Oncogene

ISSN

0950-9232

Publication Date

May 2, 1996

Volume

12

Issue

9

Start / End Page

2029 / 2034

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • RNA, Messenger
  • Oncology & Carcinogenesis
  • Humans
  • Glioma
  • Gene Rearrangement
  • Gene Deletion
  • Cyclin-Dependent Kinase Inhibitor p16
  • Carrier Proteins
  • Brain Neoplasms