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Effect of cisplatin and BCNU on MMP-2 levels in human glioblastoma cell lines in vitro.

Publication ,  Journal Article
Chintala, SK; Ali-Osman, F; Mohanam, S; Rayford, A; Go, Y; Gokaslan, ZL; Gagercas, E; Venkaiah, B; Sawaya, R; Nicolson, GL; Rao, JS
Published in: Clin Exp Metastasis
July 1997

Matrix metalloproteinases (MMPs) play an important role in various physiological and pathological conditions such as tissue remodeling, and cancer cell invasion and metastasis. The aim of this study was to determine the effect of the antitumor compounds cis-dichlorodiammine platinum (ii) (cisplatin) and 1, 3 bis (2-chloroethyl)-1-nitrosourea (BCNU) on 72-kDa type IV collagenase activity (MMP-2) in human gliomas. Human glioblastoma cell lines were treated with cisplatin (25 microM), and BCNU (50 microM), and the levels of MMP-2 were estimated in serum-free conditioned medium and in cell extracts at different time intervals. Gelatin zymography revealed increased levels of MMP-2 in serum-free conditioned medium and in cell extracts of untreated glioblastoma cell cultures during a 72-h period. In contrast, MMP-2 levels were significantly decreased in cisplatin-treated cells both in conditioned medium and cell extracts. However, no significant changes of MMP-2 levels were noted in BCNU-treated cells. Quantitative analysis of MMP-2 enzyme activity by densitometry and amount of MMP-2 protein by ELISA showed significantly decreased levels of MMP-2 in cisplatin-treated cells compared to BCNU and untreated glioblastoma cells. The results indicate that decreased levels of MMP-2 might represent an additional mechanism by which cisplatin provides its antineoplastic effects.

Duke Scholars

Published In

Clin Exp Metastasis

DOI

ISSN

0262-0898

Publication Date

July 1997

Volume

15

Issue

4

Start / End Page

361 / 367

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Oncology & Carcinogenesis
  • Metalloendopeptidases
  • Matrix Metalloproteinase 2
  • Humans
  • Glioblastoma
  • Gelatinases
  • Gelatin
  • Enzyme-Linked Immunosorbent Assay
  • Electrophoresis, Polyacrylamide Gel
 

Citation

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Chintala, S. K., Ali-Osman, F., Mohanam, S., Rayford, A., Go, Y., Gokaslan, Z. L., … Rao, J. S. (1997). Effect of cisplatin and BCNU on MMP-2 levels in human glioblastoma cell lines in vitro. Clin Exp Metastasis, 15(4), 361–367. https://doi.org/10.1023/a:1018442003163
Chintala, S. K., F. Ali-Osman, S. Mohanam, A. Rayford, Y. Go, Z. L. Gokaslan, E. Gagercas, et al. “Effect of cisplatin and BCNU on MMP-2 levels in human glioblastoma cell lines in vitro.Clin Exp Metastasis 15, no. 4 (July 1997): 361–67. https://doi.org/10.1023/a:1018442003163.
Chintala SK, Ali-Osman F, Mohanam S, Rayford A, Go Y, Gokaslan ZL, et al. Effect of cisplatin and BCNU on MMP-2 levels in human glioblastoma cell lines in vitro. Clin Exp Metastasis. 1997 Jul;15(4):361–7.
Chintala, S. K., et al. “Effect of cisplatin and BCNU on MMP-2 levels in human glioblastoma cell lines in vitro.Clin Exp Metastasis, vol. 15, no. 4, July 1997, pp. 361–67. Pubmed, doi:10.1023/a:1018442003163.
Chintala SK, Ali-Osman F, Mohanam S, Rayford A, Go Y, Gokaslan ZL, Gagercas E, Venkaiah B, Sawaya R, Nicolson GL, Rao JS. Effect of cisplatin and BCNU on MMP-2 levels in human glioblastoma cell lines in vitro. Clin Exp Metastasis. 1997 Jul;15(4):361–367.
Journal cover image

Published In

Clin Exp Metastasis

DOI

ISSN

0262-0898

Publication Date

July 1997

Volume

15

Issue

4

Start / End Page

361 / 367

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Oncology & Carcinogenesis
  • Metalloendopeptidases
  • Matrix Metalloproteinase 2
  • Humans
  • Glioblastoma
  • Gelatinases
  • Gelatin
  • Enzyme-Linked Immunosorbent Assay
  • Electrophoresis, Polyacrylamide Gel