Effect of cisplatin and BCNU on MMP-2 levels in human glioblastoma cell lines in vitro.

Journal Article (Journal Article)

Matrix metalloproteinases (MMPs) play an important role in various physiological and pathological conditions such as tissue remodeling, and cancer cell invasion and metastasis. The aim of this study was to determine the effect of the antitumor compounds cis-dichlorodiammine platinum (ii) (cisplatin) and 1, 3 bis (2-chloroethyl)-1-nitrosourea (BCNU) on 72-kDa type IV collagenase activity (MMP-2) in human gliomas. Human glioblastoma cell lines were treated with cisplatin (25 microM), and BCNU (50 microM), and the levels of MMP-2 were estimated in serum-free conditioned medium and in cell extracts at different time intervals. Gelatin zymography revealed increased levels of MMP-2 in serum-free conditioned medium and in cell extracts of untreated glioblastoma cell cultures during a 72-h period. In contrast, MMP-2 levels were significantly decreased in cisplatin-treated cells both in conditioned medium and cell extracts. However, no significant changes of MMP-2 levels were noted in BCNU-treated cells. Quantitative analysis of MMP-2 enzyme activity by densitometry and amount of MMP-2 protein by ELISA showed significantly decreased levels of MMP-2 in cisplatin-treated cells compared to BCNU and untreated glioblastoma cells. The results indicate that decreased levels of MMP-2 might represent an additional mechanism by which cisplatin provides its antineoplastic effects.

Full Text

Duke Authors

Cited Authors

  • Chintala, SK; Ali-Osman, F; Mohanam, S; Rayford, A; Go, Y; Gokaslan, ZL; Gagercas, E; Venkaiah, B; Sawaya, R; Nicolson, GL; Rao, JS

Published Date

  • July 1997

Published In

Volume / Issue

  • 15 / 4

Start / End Page

  • 361 - 367

PubMed ID

  • 9219724

Pubmed Central ID

  • 9219724

International Standard Serial Number (ISSN)

  • 0262-0898

Digital Object Identifier (DOI)

  • 10.1023/a:1018442003163


  • eng

Conference Location

  • Netherlands