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Regulation of MMP-9 (type IV collagenase) production and invasiveness in gliomas by the extracellular signal-regulated kinase and jun amino-terminal kinase signaling cascades.

Publication ,  Journal Article
Lakka, SS; Jasti, SL; Kyritsis, AP; Yung, WK; Ali-Osman, F; Nicolson, GL; Rao, JS
Published in: Clin Exp Metastasis
2000

Our previous studies have shown that MMP-9 levels are significantly elevated during the progression of human gliomas. In the current study, we examined the role of JNK- and ERK-dependent signaling modules in the regulation of MMP-9 production and the invasive behavior of the human glioblastoma cell line SNB19, in which JNK/ERK1 is constitutively activated. SNB19 cells that were transfected with dominant-negative JNK, MEKK, and ERK1 expression vectors showed reduced MMP-9 promoter activity. In addition, conditioned medium collected from SNB19 cells transfected with these expression vectors showed diminished MMP-9 activity in the presence of phorbol myristate acetate, as determined by gelatin zymography. The cotransfection of SNB19 cells with kinase-deficient c-raf also diminished MMP-9 promoter activity. Further, in the presence of a specific inhibitor of MEKK (PD098059), the Matrigel invasion assay showed the invasiveness of dominant-negative SNB19 cells transfected with dominant-negative JNK1 or ERK1 to be remarkably reduced. In conclusion, our studies showed for the first time that MMP-9 production and the invasive behavior of SNB 19 cells are regulated by JNK- and ERK-dependent signaling modules and that interfering with either of the pathways reduces invasiveness.

Duke Scholars

Published In

Clin Exp Metastasis

DOI

ISSN

0262-0898

Publication Date

2000

Volume

18

Issue

3

Start / End Page

245 / 252

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcriptional Activation
  • Signal Transduction
  • Promoter Regions, Genetic
  • Oncology & Carcinogenesis
  • Mitogen-Activated Protein Kinases
  • Mitogen-Activated Protein Kinase 3
  • Matrix Metalloproteinase 9
  • JNK Mitogen-Activated Protein Kinases
  • Humans
 

Citation

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ICMJE
MLA
NLM
Lakka, S. S., Jasti, S. L., Kyritsis, A. P., Yung, W. K., Ali-Osman, F., Nicolson, G. L., & Rao, J. S. (2000). Regulation of MMP-9 (type IV collagenase) production and invasiveness in gliomas by the extracellular signal-regulated kinase and jun amino-terminal kinase signaling cascades. Clin Exp Metastasis, 18(3), 245–252. https://doi.org/10.1023/a:1006724826083
Lakka, S. S., S. L. Jasti, A. P. Kyritsis, W. K. Yung, F. Ali-Osman, G. L. Nicolson, and J. S. Rao. “Regulation of MMP-9 (type IV collagenase) production and invasiveness in gliomas by the extracellular signal-regulated kinase and jun amino-terminal kinase signaling cascades.Clin Exp Metastasis 18, no. 3 (2000): 245–52. https://doi.org/10.1023/a:1006724826083.
Lakka SS, Jasti SL, Kyritsis AP, Yung WK, Ali-Osman F, Nicolson GL, et al. Regulation of MMP-9 (type IV collagenase) production and invasiveness in gliomas by the extracellular signal-regulated kinase and jun amino-terminal kinase signaling cascades. Clin Exp Metastasis. 2000;18(3):245–52.
Lakka, S. S., et al. “Regulation of MMP-9 (type IV collagenase) production and invasiveness in gliomas by the extracellular signal-regulated kinase and jun amino-terminal kinase signaling cascades.Clin Exp Metastasis, vol. 18, no. 3, 2000, pp. 245–52. Pubmed, doi:10.1023/a:1006724826083.
Lakka SS, Jasti SL, Kyritsis AP, Yung WK, Ali-Osman F, Nicolson GL, Rao JS. Regulation of MMP-9 (type IV collagenase) production and invasiveness in gliomas by the extracellular signal-regulated kinase and jun amino-terminal kinase signaling cascades. Clin Exp Metastasis. 2000;18(3):245–252.
Journal cover image

Published In

Clin Exp Metastasis

DOI

ISSN

0262-0898

Publication Date

2000

Volume

18

Issue

3

Start / End Page

245 / 252

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcriptional Activation
  • Signal Transduction
  • Promoter Regions, Genetic
  • Oncology & Carcinogenesis
  • Mitogen-Activated Protein Kinases
  • Mitogen-Activated Protein Kinase 3
  • Matrix Metalloproteinase 9
  • JNK Mitogen-Activated Protein Kinases
  • Humans