High-throughput detection of glutathione s-transferase polymorphic alleles in a pediatric cancer population.

Journal Article (Clinical Trial;Journal Article)

Polymorphisms of glutathione S-transferase (GST) enzymes have been correlated with altered risk of several cancers, as well as altered response and toxicity from cancer chemotherapy. We report a low cost, highly reproducible and specific PCR-based high-throughput assay for genotyping different GSTs designed for use in large clinical trials. In comparison to an alternative genotyping method (single nucleotide extension), the sensitivity and specificity of the high throughput assay was shown to be 92 and 97%, respectively, depending on the source of genomic DNA. Using the high-throughput assay, we demonstrate by multivariate analysis an increased risk of acute lymphoblastic leukemia, glial brain tumors, and osteosarcoma for patients carrying nonnull alleles of GSTM1 and/or GSTT1.

Full Text

Duke Authors

Cited Authors

  • Barnette, P; Scholl, R; Blandford, M; Ballard, L; Tsodikov, A; Magee, J; Williams, S; Robertson, M; Ali-Osman, F; Lemons, R; Keller, C

Published Date

  • February 2004

Published In

Volume / Issue

  • 13 / 2

Start / End Page

  • 304 - 313

PubMed ID

  • 14973099

International Standard Serial Number (ISSN)

  • 1055-9965

Digital Object Identifier (DOI)

  • 10.1158/1055-9965.epi-03-0178


  • eng

Conference Location

  • United States