Skip to main content
Journal cover image

Transfection of cells with transforming growth factor-alpha leads to cellular resistance to the antiproliferative effects of tumor necrosis factor.

Publication ,  Journal Article
Aggarwal, BB; Pocsik, E; Ali-Osman, F; Totpal, K
Published in: FEBS Lett
October 31, 1994

Tumor necrosis factor (TNF) is a growth-modulatory cytokine that inhibits the growth of certain cell lines, stimulates the growth of some, and has no effect on the growth of still others. The molecular basis for this differential regulation of growth by TNF is not understood. We postulate that the growth of normal or tumor cells is determined by the balance between growth-stimulatory and -inhibitory signals. In the present study, we demonstrate that the transfection of cells with the transforming growth factor (TGF)-alpha gene induces resistance to TNF. Colon carcinoma cell lines that express elevated levels of TGF-alpha were also found to be resistant to this cytokine. Exogenous addition of the growth factor was also effective in decreasing the antiproliferative effects of TNF. Transfection of cells with the TGF-alpha gene led to downmodulation of TNF receptors but an increase in intracellular glutathione levels. Thus, these results support our hypothesis that expression of growth factors by certain tumor cells can lead to resistance to antiproliferative agents such as TNF.

Duke Scholars

Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

October 31, 1994

Volume

354

Issue

1

Start / End Page

12 / 16

Location

England

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Tumor Cells, Cultured
  • Transforming Growth Factor alpha
  • Transfection
  • Receptors, Tumor Necrosis Factor
  • Mice
  • Humans
  • Glutathione
  • Down-Regulation
  • Cell Division
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Aggarwal, B. B., Pocsik, E., Ali-Osman, F., & Totpal, K. (1994). Transfection of cells with transforming growth factor-alpha leads to cellular resistance to the antiproliferative effects of tumor necrosis factor. FEBS Lett, 354(1), 12–16. https://doi.org/10.1016/0014-5793(94)01073-0
Aggarwal, B. B., E. Pocsik, F. Ali-Osman, and K. Totpal. “Transfection of cells with transforming growth factor-alpha leads to cellular resistance to the antiproliferative effects of tumor necrosis factor.FEBS Lett 354, no. 1 (October 31, 1994): 12–16. https://doi.org/10.1016/0014-5793(94)01073-0.
Aggarwal, B. B., et al. “Transfection of cells with transforming growth factor-alpha leads to cellular resistance to the antiproliferative effects of tumor necrosis factor.FEBS Lett, vol. 354, no. 1, Oct. 1994, pp. 12–16. Pubmed, doi:10.1016/0014-5793(94)01073-0.
Journal cover image

Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

October 31, 1994

Volume

354

Issue

1

Start / End Page

12 / 16

Location

England

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Tumor Cells, Cultured
  • Transforming Growth Factor alpha
  • Transfection
  • Receptors, Tumor Necrosis Factor
  • Mice
  • Humans
  • Glutathione
  • Down-Regulation
  • Cell Division