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Suppression of antiproliferative effects of tumor necrosis factor by transfection of cells with human platelet-derived growth factor B/c-sis gene.

Publication ,  Journal Article
Aggarwal, BB; Pocsik, E; Totpal, K; Ali-Osman, F
Published in: FEBS Lett
January 2, 1995

The growth of cells is determined by the balance between growth-stimulatory and growth-inhibitory signals. In the present study, we demonstrate that the transfection of NIH 3T3 cells with a platelet-derived growth factor (PDGF-Blc-sis) gene induces resistance to the anticellular effects of tumor necrosis factor (TNF). Human tumor cell lines that express elevated levels of c-sis (e.g. epidermoid carcinoma, A-431) are also TNF resistant, whereas those that express no significant levels of this gene (e.g. breast adenocarcinoma, MCF-7) are TNF sensitive. Transfection of cells with the c-sis gene leads to down-modulation of TNF receptors and also a decrease in intracellular glutathione levels. Thus, our results demonstrate that over-expression of PDGF-Blc-sis by certain tumor cells can lead to their protection from the anticellular effects of TNF.

Duke Scholars

Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

January 2, 1995

Volume

357

Issue

1

Start / End Page

1 / 6

Location

England

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Tumor Cells, Cultured
  • Transfection
  • Receptors, Tumor Necrosis Factor
  • Proto-Oncogene Proteins c-sis
  • Proto-Oncogene Proteins
  • Platelet-Derived Growth Factor
  • Mice
  • Interferon-gamma
  • Humans
 

Citation

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Aggarwal, B. B., Pocsik, E., Totpal, K., & Ali-Osman, F. (1995). Suppression of antiproliferative effects of tumor necrosis factor by transfection of cells with human platelet-derived growth factor B/c-sis gene. FEBS Lett, 357(1), 1–6. https://doi.org/10.1016/0014-5793(94)01299-g
Aggarwal, B. B., E. Pocsik, K. Totpal, and F. Ali-Osman. “Suppression of antiproliferative effects of tumor necrosis factor by transfection of cells with human platelet-derived growth factor B/c-sis gene.FEBS Lett 357, no. 1 (January 2, 1995): 1–6. https://doi.org/10.1016/0014-5793(94)01299-g.
Aggarwal, B. B., et al. “Suppression of antiproliferative effects of tumor necrosis factor by transfection of cells with human platelet-derived growth factor B/c-sis gene.FEBS Lett, vol. 357, no. 1, Jan. 1995, pp. 1–6. Pubmed, doi:10.1016/0014-5793(94)01299-g.
Journal cover image

Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

January 2, 1995

Volume

357

Issue

1

Start / End Page

1 / 6

Location

England

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Tumor Cells, Cultured
  • Transfection
  • Receptors, Tumor Necrosis Factor
  • Proto-Oncogene Proteins c-sis
  • Proto-Oncogene Proteins
  • Platelet-Derived Growth Factor
  • Mice
  • Interferon-gamma
  • Humans