Suppression of antiproliferative effects of tumor necrosis factor by transfection of cells with human platelet-derived growth factor B/c-sis gene.

Journal Article

The growth of cells is determined by the balance between growth-stimulatory and growth-inhibitory signals. In the present study, we demonstrate that the transfection of NIH 3T3 cells with a platelet-derived growth factor (PDGF-Blc-sis) gene induces resistance to the anticellular effects of tumor necrosis factor (TNF). Human tumor cell lines that express elevated levels of c-sis (e.g. epidermoid carcinoma, A-431) are also TNF resistant, whereas those that express no significant levels of this gene (e.g. breast adenocarcinoma, MCF-7) are TNF sensitive. Transfection of cells with the c-sis gene leads to down-modulation of TNF receptors and also a decrease in intracellular glutathione levels. Thus, our results demonstrate that over-expression of PDGF-Blc-sis by certain tumor cells can lead to their protection from the anticellular effects of TNF.

Full Text

Duke Authors

Cited Authors

  • Aggarwal, BB; Pocsik, E; Totpal, K; Ali-Osman, F

Published Date

  • January 2, 1995

Published In

Volume / Issue

  • 357 / 1

Start / End Page

  • 1 - 6

PubMed ID

  • 8001667

International Standard Serial Number (ISSN)

  • 0014-5793

Language

  • eng

Conference Location

  • England