Two different types of granulocyte colony-stimulating factor produced by bovine lung tissue.

Journal Article (Journal Article)

Bovine lung tissue produces two different types of granulocyte colony-stimulating factor (CSF). The high molecular weight (MW) type (CSF-F) of 70,000 d by Sephadex G-100 gel filtration is only found in conditioned medium of homogenized tissue incubated in sealed glass bottles. The species of CSF exclusively stimulates CFU-C of mouse bone marrow, human bone marrow only hardly. The low MW type CSF (CSF-M) of approximately 29,000 d by gel filtration is found mainly in conditioned medium of slightly minced tissue incubated in Petri dishes. It stimulates both human and mouse CFU-C. Methods to prepare both types of CSF are described. By propagating a fibroblast cell line from bovine lung tissue it was found that fibroblasts are the source of the 70,000 d CSF. Indirect evidence suggests that macrophages produce the 29,000 d CSF species.

Full Text

Duke Authors

Cited Authors

  • Neumeier, R; Ali-Osman, F; Maurer, HR

Published Date

  • January 1, 1982

Published In

Volume / Issue

  • 44 / 1

Start / End Page

  • 21 - 27

PubMed ID

  • 6977391

International Standard Serial Number (ISSN)

  • 0006-5242

Digital Object Identifier (DOI)

  • 10.1007/BF00320683


  • eng

Conference Location

  • Germany