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Cisplatin but not BCNU inhibits urokinase-type plasminogen activator levels in human glioblastoma cell lines in vitro.

Publication ,  Journal Article
Go, Y; Chintala, SK; Rayford, A; Gagercas, E; Ali-Osman, F; Venkaiah, B; Sawaya, R; Gokaslan, Z; Nicolson, GL; Rao, JS
Published in: Clin Exp Metastasis
July 1997

Glioblastomas extensively invade the surrounding normal brain tissue, with a concomitant expression of various proteolytic enzymes, in particular urokinase-type plasminogen activator (uPA). In this study we used cis-diamminedichloroplatinum (cisplatin) and 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), commonly used anti-cancer drugs for the treatment of glioblastomas, to study the expression of uPA in three human glioblastoma cell lines in vitro. Cells were treated with 25 microM cisplatin and 50 microM BCNU, and uPA levels were estimated by fibrin zymography during a 72-h time course. Treatment of glioblastoma cells with cisplatin resulted in significantly decreased levels of uPA in serum-free conditioned medium and cell extracts, compared to BCNU-treated and untreated cell lines. Quantitative levels of uPA enzyme activity assessed by scanning laser densitometry and uPA protein by ELISA using antibody against uPA showed decreased levels of uPA in cisplatin-treated glioma cell lines relative to BCNU and untreated cell lines. Our results suggest that anti-tumor compound, cisplatin, may exert its anti-neoplastic effects by inhibiting uPA in malignant glioblastomas.

Duke Scholars

Published In

Clin Exp Metastasis

DOI

ISSN

0262-0898

Publication Date

July 1997

Volume

15

Issue

4

Start / End Page

447 / 452

Location

Netherlands

Related Subject Headings

  • Urokinase-Type Plasminogen Activator
  • Tumor Cells, Cultured
  • Oncology & Carcinogenesis
  • Neoplasm Invasiveness
  • Humans
  • Glioblastoma
  • Fibrin
  • Enzyme-Linked Immunosorbent Assay
  • Electrophoresis, Polyacrylamide Gel
  • Culture Media, Serum-Free
 

Citation

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Go, Y., Chintala, S. K., Rayford, A., Gagercas, E., Ali-Osman, F., Venkaiah, B., … Rao, J. S. (1997). Cisplatin but not BCNU inhibits urokinase-type plasminogen activator levels in human glioblastoma cell lines in vitro. Clin Exp Metastasis, 15(4), 447–452. https://doi.org/10.1023/a:1018462507706
Go, Y., S. K. Chintala, A. Rayford, E. Gagercas, F. Ali-Osman, B. Venkaiah, R. Sawaya, Z. Gokaslan, G. L. Nicolson, and J. S. Rao. “Cisplatin but not BCNU inhibits urokinase-type plasminogen activator levels in human glioblastoma cell lines in vitro.Clin Exp Metastasis 15, no. 4 (July 1997): 447–52. https://doi.org/10.1023/a:1018462507706.
Go Y, Chintala SK, Rayford A, Gagercas E, Ali-Osman F, Venkaiah B, et al. Cisplatin but not BCNU inhibits urokinase-type plasminogen activator levels in human glioblastoma cell lines in vitro. Clin Exp Metastasis. 1997 Jul;15(4):447–52.
Go, Y., et al. “Cisplatin but not BCNU inhibits urokinase-type plasminogen activator levels in human glioblastoma cell lines in vitro.Clin Exp Metastasis, vol. 15, no. 4, July 1997, pp. 447–52. Pubmed, doi:10.1023/a:1018462507706.
Go Y, Chintala SK, Rayford A, Gagercas E, Ali-Osman F, Venkaiah B, Sawaya R, Gokaslan Z, Nicolson GL, Rao JS. Cisplatin but not BCNU inhibits urokinase-type plasminogen activator levels in human glioblastoma cell lines in vitro. Clin Exp Metastasis. 1997 Jul;15(4):447–452.
Journal cover image

Published In

Clin Exp Metastasis

DOI

ISSN

0262-0898

Publication Date

July 1997

Volume

15

Issue

4

Start / End Page

447 / 452

Location

Netherlands

Related Subject Headings

  • Urokinase-Type Plasminogen Activator
  • Tumor Cells, Cultured
  • Oncology & Carcinogenesis
  • Neoplasm Invasiveness
  • Humans
  • Glioblastoma
  • Fibrin
  • Enzyme-Linked Immunosorbent Assay
  • Electrophoresis, Polyacrylamide Gel
  • Culture Media, Serum-Free