Rapid method for permanent slide preparation of colonies in soft agar cultures.
Published
Journal Article
A method is presented for preparing permanent microscopic slides from colony-bearing agar layers in soft agar cultures. The main advantages of this technique are its simplicity, rapidity and accurate colony preservation. This method could have broad applications in the human tumor clonogenic assay (HTCA), particularly in the quantitative morphological, cytochemical and immunocytochemical assessment of colonies that form in both control and drug-treated cultures. Thus, this method opens up possibilities for using cytopathological criteria as a quantitative endpoint of the HTCA.
Full Text
Duke Authors
Cited Authors
- Moezzi, J; Ali-Osman, F; Murphy, MJ
Published Date
- September 1986
Published In
Volume / Issue
- 4 / 5
Start / End Page
- 368 - 374
PubMed ID
- 3534111
Pubmed Central ID
- 3534111
International Standard Serial Number (ISSN)
- 0737-1454
Digital Object Identifier (DOI)
- 10.1002/stem.5530040508
Language
- eng
Conference Location
- United States