Rapid method for permanent slide preparation of colonies in soft agar cultures.


Journal Article

A method is presented for preparing permanent microscopic slides from colony-bearing agar layers in soft agar cultures. The main advantages of this technique are its simplicity, rapidity and accurate colony preservation. This method could have broad applications in the human tumor clonogenic assay (HTCA), particularly in the quantitative morphological, cytochemical and immunocytochemical assessment of colonies that form in both control and drug-treated cultures. Thus, this method opens up possibilities for using cytopathological criteria as a quantitative endpoint of the HTCA.

Full Text

Duke Authors

Cited Authors

  • Moezzi, J; Ali-Osman, F; Murphy, MJ

Published Date

  • September 1986

Published In

Volume / Issue

  • 4 / 5

Start / End Page

  • 368 - 374

PubMed ID

  • 3534111

Pubmed Central ID

  • 3534111

International Standard Serial Number (ISSN)

  • 0737-1454

Digital Object Identifier (DOI)

  • 10.1002/stem.5530040508


  • eng

Conference Location

  • United States