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Estrogen-induced mitogenesis of MCF-7 cells does not require the induction of mitogen-activated protein kinase activity.

Publication ,  Journal Article
Lobenhofer, EK; Marks, JR
Published in: J Steroid Biochem Mol Biol
December 1, 2000

Estrogen mediates the transcription of responsive genes via its interaction with the estrogen receptor (ER). This ligand-dependent transcriptional activity has been the mechanistic basis for understanding estrogen-induced proliferation. However, recent reports suggest that estrogen stimulation results in activation of the mitogen-activated protein kinase (MAPK) cascade in an ER-dependent manner suggesting that mitogenesis may be mediated through this cytoplasmic signaling cascade. In this study, we demonstrate that estrogen stimulation of MCF-7 cells does not activate MAPK regardless of hormone concentration, serum concentration, or cell density. We also excluded the activation of MAPK through autocrine effects after estrogen treatment. Finally, concentrations required for estrogen-induced mitogenesis and estrogen-mediated transcription were shown to be the same. These results support transcriptional activation as the primary mechanism for estrogen-mediated mitogenesis.

Duke Scholars

Published In

J Steroid Biochem Mol Biol

DOI

ISSN

0960-0760

Publication Date

December 1, 2000

Volume

75

Issue

1

Start / End Page

11 / 20

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Transcription, Genetic
  • Receptors, Estrogen
  • RNA, Messenger
  • Phosphorylation
  • Mitogens
  • Mitogen-Activated Protein Kinases
  • Mitogen-Activated Protein Kinase 3
  • Mitogen-Activated Protein Kinase 1
 

Citation

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Lobenhofer, E. K., & Marks, J. R. (2000). Estrogen-induced mitogenesis of MCF-7 cells does not require the induction of mitogen-activated protein kinase activity. J Steroid Biochem Mol Biol, 75(1), 11–20. https://doi.org/10.1016/s0960-0760(00)00132-1
Lobenhofer, E. K., and J. R. Marks. “Estrogen-induced mitogenesis of MCF-7 cells does not require the induction of mitogen-activated protein kinase activity.J Steroid Biochem Mol Biol 75, no. 1 (December 1, 2000): 11–20. https://doi.org/10.1016/s0960-0760(00)00132-1.
Lobenhofer, E. K., and J. R. Marks. “Estrogen-induced mitogenesis of MCF-7 cells does not require the induction of mitogen-activated protein kinase activity.J Steroid Biochem Mol Biol, vol. 75, no. 1, Dec. 2000, pp. 11–20. Pubmed, doi:10.1016/s0960-0760(00)00132-1.
Journal cover image

Published In

J Steroid Biochem Mol Biol

DOI

ISSN

0960-0760

Publication Date

December 1, 2000

Volume

75

Issue

1

Start / End Page

11 / 20

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Transcription, Genetic
  • Receptors, Estrogen
  • RNA, Messenger
  • Phosphorylation
  • Mitogens
  • Mitogen-Activated Protein Kinases
  • Mitogen-Activated Protein Kinase 3
  • Mitogen-Activated Protein Kinase 1