Estrogen-induced mitogenesis of MCF-7 cells does not require the induction of mitogen-activated protein kinase activity.

Published

Journal Article

Estrogen mediates the transcription of responsive genes via its interaction with the estrogen receptor (ER). This ligand-dependent transcriptional activity has been the mechanistic basis for understanding estrogen-induced proliferation. However, recent reports suggest that estrogen stimulation results in activation of the mitogen-activated protein kinase (MAPK) cascade in an ER-dependent manner suggesting that mitogenesis may be mediated through this cytoplasmic signaling cascade. In this study, we demonstrate that estrogen stimulation of MCF-7 cells does not activate MAPK regardless of hormone concentration, serum concentration, or cell density. We also excluded the activation of MAPK through autocrine effects after estrogen treatment. Finally, concentrations required for estrogen-induced mitogenesis and estrogen-mediated transcription were shown to be the same. These results support transcriptional activation as the primary mechanism for estrogen-mediated mitogenesis.

Full Text

Duke Authors

Cited Authors

  • Lobenhofer, EK; Marks, JR

Published Date

  • December 1, 2000

Published In

Volume / Issue

  • 75 / 1

Start / End Page

  • 11 - 20

PubMed ID

  • 11179904

Pubmed Central ID

  • 11179904

International Standard Serial Number (ISSN)

  • 0960-0760

Digital Object Identifier (DOI)

  • 10.1016/s0960-0760(00)00132-1

Language

  • eng

Conference Location

  • England