Molecular cloning and restriction endonuclease mapping of the murine cytomegalovirus genome (Smith Strain).

Published

Journal Article

We have cloned EcoRI and HindIII fragments of the Smith strain of murine cytomegalovirus (MCMV) in the plasmid vector pACYC184. These cloned fragments were used to establish a restriction endonuclease map of the genome with respect to the EcoRI and HindIII sites. The map was constructed on the basis of data derived from cross-hybridizations of EcoRI and HindIII cloned fragments, double-digestions of the cloned fragments with EcoRI and HindIII, and hybridization of cloned HindIII fragments to Southern blots of MCMV DNA cleaved with EcoRI. From our mapping data, we have determined that the length of the MCMV genome is approximately 240 kbp. The genome does not appear to undergo inversions and lacks detectable repeated sequences. One HindIII cloned fragment was obtained which contained both HindIII termini. The existence of this fragment may be related to the mode of replication of the MCMV genome.

Full Text

Duke Authors

Cited Authors

  • Mercer, JA; Marks, JR; Spector, DH

Published Date

  • August 1983

Published In

Volume / Issue

  • 129 / 1

Start / End Page

  • 94 - 106

PubMed ID

  • 6310888

Pubmed Central ID

  • 6310888

International Standard Serial Number (ISSN)

  • 0042-6822

Digital Object Identifier (DOI)

  • 10.1016/0042-6822(83)90398-7

Language

  • eng

Conference Location

  • United States