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Repression of interleukin-2 mRNA translation in primary human breast carcinoma tumor-infiltrating lymphocytes.

Publication ,  Journal Article
Lopez, CB; Rao, TD; Feiner, H; Shapiro, R; Marks, JR; Frey, AB
Published in: Cell Immunol
December 15, 1998

Human breast carcinoma tumor-infiltrating lymphocytes (TIL) express activation antigens in situ indicative of ongoing immune response-CD28, CD45RO, CD69, CD71, and DR. However, interleukin 2 (IL-2) receptor was poorly expressed: CD25 was detected in only 1/24 samples and CD122 in only 2/24 samples. Furthermore, isolated breast cancer TIL were defective in proliferative response but recover when treated with recombinant IL-2. Nineteen of 24 tumor samples expressed B7-1, B7-2, and CD28 protein, showing that absence of costimulator proteins or counter ligand was not the basis for TIL proliferative deficit. Expression of IL-2 activity was not detected; however, mRNA encoding IL-2 was produced and translatable in vitro. These findings show that human breast cancer tumor-induced repression of IL-2 RNA translation is the basis of failure of TIL to express the IL-2 receptor and subsequent T cell hyporesponsiveness.

Duke Scholars

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

December 15, 1998

Volume

190

Issue

2

Start / End Page

141 / 155

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Receptors, Interleukin-2
  • RNA, Messenger
  • Protein Biosynthesis
  • Lymphocytes, Tumor-Infiltrating
  • Interleukin-2
  • Immunology
  • Humans
  • Gene Expression Regulation
  • Female
 

Citation

APA
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ICMJE
MLA
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Lopez, C. B., Rao, T. D., Feiner, H., Shapiro, R., Marks, J. R., & Frey, A. B. (1998). Repression of interleukin-2 mRNA translation in primary human breast carcinoma tumor-infiltrating lymphocytes. Cell Immunol, 190(2), 141–155. https://doi.org/10.1006/cimm.1998.1390
Lopez, C. B., T. D. Rao, H. Feiner, R. Shapiro, J. R. Marks, and A. B. Frey. “Repression of interleukin-2 mRNA translation in primary human breast carcinoma tumor-infiltrating lymphocytes.Cell Immunol 190, no. 2 (December 15, 1998): 141–55. https://doi.org/10.1006/cimm.1998.1390.
Lopez CB, Rao TD, Feiner H, Shapiro R, Marks JR, Frey AB. Repression of interleukin-2 mRNA translation in primary human breast carcinoma tumor-infiltrating lymphocytes. Cell Immunol. 1998 Dec 15;190(2):141–55.
Lopez, C. B., et al. “Repression of interleukin-2 mRNA translation in primary human breast carcinoma tumor-infiltrating lymphocytes.Cell Immunol, vol. 190, no. 2, Dec. 1998, pp. 141–55. Pubmed, doi:10.1006/cimm.1998.1390.
Lopez CB, Rao TD, Feiner H, Shapiro R, Marks JR, Frey AB. Repression of interleukin-2 mRNA translation in primary human breast carcinoma tumor-infiltrating lymphocytes. Cell Immunol. 1998 Dec 15;190(2):141–155.
Journal cover image

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

December 15, 1998

Volume

190

Issue

2

Start / End Page

141 / 155

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Receptors, Interleukin-2
  • RNA, Messenger
  • Protein Biosynthesis
  • Lymphocytes, Tumor-Infiltrating
  • Interleukin-2
  • Immunology
  • Humans
  • Gene Expression Regulation
  • Female