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TAFII70 isoform-specific growth suppression correlates with its ability to complex with the GADD45a protein.

Publication ,  Journal Article
Wang, W; Nahta, R; Huper, G; Marks, JR
Published in: Mol Cancer Res
August 2004

TAFII70, a member of the basal transcription complex implicated in p53-mediated transcription, is synthesized as several alternately spliced variants. The predominant forms found in normal and neoplastic breast epithelial cells are shown to be 72 kDa (TAFII70) and 78 kDa (TAFII80). Most cancers express higher levels of the TAFII80 isoform, whereas normal breast epithelia express higher levels of the TAFII70 isoform. Expression of TAFII70, but not TAFII80, causes dramatic growth suppression of normal and transformed breast epithelial cell lines in a p53-independent manner. Growth suppression correlates with mitotic inhibition resulting from an increased number of cells in G2. Both isoforms induce expression of the G2 arrest associated gene, GADD45a, but a novel protein-protein interaction was observed between TAFII70 (not TAFII80) and GADD45a, suggesting that this interaction is important for the observed growth arrest phenotype induced by the TAFII70 isoform. GADD45a null cells are not subject to TAFII70 inhibition, further supporting the relevance of this interaction.

Duke Scholars

Published In

Mol Cancer Res

ISSN

1541-7786

Publication Date

August 2004

Volume

2

Issue

8

Start / End Page

442 / 452

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Transcription, Genetic
  • Transcription Factors
  • TATA-Binding Protein Associated Factors
  • Protein Isoforms
  • Protein Binding
  • Oncology & Carcinogenesis
  • Nuclear Proteins
  • Mutation
  • Molecular Sequence Data
 

Citation

APA
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ICMJE
MLA
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Wang, W., Nahta, R., Huper, G., & Marks, J. R. (2004). TAFII70 isoform-specific growth suppression correlates with its ability to complex with the GADD45a protein. Mol Cancer Res, 2(8), 442–452.
Wang, Wei, Rita Nahta, Gudrun Huper, and Jeffrey R. Marks. “TAFII70 isoform-specific growth suppression correlates with its ability to complex with the GADD45a protein.Mol Cancer Res 2, no. 8 (August 2004): 442–52.
Wang W, Nahta R, Huper G, Marks JR. TAFII70 isoform-specific growth suppression correlates with its ability to complex with the GADD45a protein. Mol Cancer Res. 2004 Aug;2(8):442–52.
Wang, Wei, et al. “TAFII70 isoform-specific growth suppression correlates with its ability to complex with the GADD45a protein.Mol Cancer Res, vol. 2, no. 8, Aug. 2004, pp. 442–52.
Wang W, Nahta R, Huper G, Marks JR. TAFII70 isoform-specific growth suppression correlates with its ability to complex with the GADD45a protein. Mol Cancer Res. 2004 Aug;2(8):442–452.

Published In

Mol Cancer Res

ISSN

1541-7786

Publication Date

August 2004

Volume

2

Issue

8

Start / End Page

442 / 452

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Transcription, Genetic
  • Transcription Factors
  • TATA-Binding Protein Associated Factors
  • Protein Isoforms
  • Protein Binding
  • Oncology & Carcinogenesis
  • Nuclear Proteins
  • Mutation
  • Molecular Sequence Data