Genomic amplification and oncogenic properties of the KCNK9 potassium channel gene.
Representational difference analysis (RDA) of human breast cancer was used to discover a novel amplicon located at chromosomal region 8q24.3. We examined a series of breast cancer samples harboring amplification of this region and determined that KCNK9 is the sole overexpressed gene within the amplification epicenter. KCNK9 encodes a potassium channel that is amplified from 3-fold to 10-fold in 10% of breast tumors and overexpressed from 5-fold to over 100-fold in 44% of breast tumors. Overexpression of KCNK9 in cell lines promotes tumor formation and confers resistance to both hypoxia and serum deprivation, suggesting that its amplification and overexpression plays a physiologically important role in human breast cancer.
Mu, D; Chen, L; Zhang, X; See, L-H; Koch, CM; Yen, C; Tong, JJ; Spiegel, L; Nguyen, KCQ; Servoss, A; Peng, Y; Pei, L; Marks, JR; Lowe, S; Hoey, T; Jan, LY; McCombie, WR; Wigler, MH; Powers, S
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