Skip to main content
Journal cover image

Meta-[131I]iodobenzylguanidine uptake and meta-[211At]astatobenzylguanidine treatment in human medulloblastoma cell lines.

Publication ,  Journal Article
Strickland, DK; Vaidyanathan, G; Friedman, HS; Zalutsky, MR
Published in: J Neurooncol
1995

Uptake of radioiodinated meta-iodobenzylguanidine (MIBG) has been demonstrated in the neural crest tumors, including neuroblastoma, pheochromocytoma, and carcinoid tumors, and is presently in use diagnostically and therapeutically in these settings. Cells comprising medulloblastoma, the most common central nervous system malignancy in childhood, may be derived from a common germinal neuroepithelial cell as neural crest tissue, and as a result, also may have the capacity for accumulating MIBG. To investigate this hypothesis, we measured the in vitro binding of [131I]MIBG to 9 medulloblastoma-derived cell lines and the SK-N-SH neuroblastoma line known to accumulate MIBG. Seven of the medulloblastoma lines exhibited MIBG binding. The cell line with the greatest uptake, D384 Med, bound 11.2 +/- 0.9% of added [131I]MIBG activity compared with 47.1 +/- 2.3% for the SK-N-SH cell line. When 2 of the cell lines, D384 Med and D458 Med, were treated with the alpha-particle emitting analogue meta-[211At]astatobenzylguanidine ([211At]MABG), as much as a 3-log cell kill was observed in limiting dilution clonogenic assays. Exposure to considerably higher activity levels of [211At]astatide was required to achieve a similar degree of cell kill, suggesting that this cytotoxicity was not related to nonspecific effects of alpha-particle irradiation. We conclude that the uptake capacity of medulloblastoma cell lines for [131I]MIBG uptake in vitro, while lower than that seen in SK-N-SH neuroblastoma cells, is sufficient to permit [211At]MABG to be used with significant therapeutic effectiveness.

Duke Scholars

Published In

J Neurooncol

DOI

ISSN

0167-594X

Publication Date

1995

Volume

25

Issue

1

Start / End Page

9 / 17

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Oncology & Carcinogenesis
  • Neuroblastoma
  • Medulloblastoma
  • Kinetics
  • Iodobenzenes
  • Iodine Radioisotopes
  • Humans
  • Guanidines
  • Cerebellar Neoplasms
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Strickland, D. K., Vaidyanathan, G., Friedman, H. S., & Zalutsky, M. R. (1995). Meta-[131I]iodobenzylguanidine uptake and meta-[211At]astatobenzylguanidine treatment in human medulloblastoma cell lines. J Neurooncol, 25(1), 9–17. https://doi.org/10.1007/BF01054718
Strickland, D. K., G. Vaidyanathan, H. S. Friedman, and M. R. Zalutsky. “Meta-[131I]iodobenzylguanidine uptake and meta-[211At]astatobenzylguanidine treatment in human medulloblastoma cell lines.J Neurooncol 25, no. 1 (1995): 9–17. https://doi.org/10.1007/BF01054718.
Strickland DK, Vaidyanathan G, Friedman HS, Zalutsky MR. Meta-[131I]iodobenzylguanidine uptake and meta-[211At]astatobenzylguanidine treatment in human medulloblastoma cell lines. J Neurooncol. 1995;25(1):9–17.
Strickland, D. K., et al. “Meta-[131I]iodobenzylguanidine uptake and meta-[211At]astatobenzylguanidine treatment in human medulloblastoma cell lines.J Neurooncol, vol. 25, no. 1, 1995, pp. 9–17. Pubmed, doi:10.1007/BF01054718.
Strickland DK, Vaidyanathan G, Friedman HS, Zalutsky MR. Meta-[131I]iodobenzylguanidine uptake and meta-[211At]astatobenzylguanidine treatment in human medulloblastoma cell lines. J Neurooncol. 1995;25(1):9–17.
Journal cover image

Published In

J Neurooncol

DOI

ISSN

0167-594X

Publication Date

1995

Volume

25

Issue

1

Start / End Page

9 / 17

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Oncology & Carcinogenesis
  • Neuroblastoma
  • Medulloblastoma
  • Kinetics
  • Iodobenzenes
  • Iodine Radioisotopes
  • Humans
  • Guanidines
  • Cerebellar Neoplasms