C1-esterase inhibitor and a novel peptide inhibitor improve contractile function in reperfused skeletal muscle.

Journal Article (Journal Article)

To determine the role of inhibition of complement activation in the contractile function of skeletal muscle ischemia-reperfusion (I/R) injury, the rat extensor digitorum longus (EDL) muscles underwent 3 h ischemia and received human C1-esterase inhibitor (C1-INH, 100 IU/kg), a synthetic C1q A chain peptide with a similar inhibitory effect on activated C1 (peptide, 5 mg/kg), or human serum albumin control. Results showed a significant overall increase in tetanic contractile forces of the reperfused EDL in both C1-INH and peptide groups compared to controls. Maximum improvement occurred with peptide treatment at 120-Hz stimulation, with an increase in force from 38 +/- 4% of normal in controls to 52 +/- 4% in peptide-treated rats. There were no significant differences between C1-INH and peptide groups. Plasma C3 and C4 activities were significantly increased in both treated groups, suggesting inhibition of complement activation. Our results suggest that complement activation is involved in I/R injury, and inhibition of complement activation may therefore represent a potential therapeutic approach to reducing or preventing I/R injury.

Full Text

Duke Authors

Cited Authors

  • Toomayan, GA; Chen, L-E; Jiang, H-X; Qi, W-N; Seaber, AV; Frank, MM; Urbaniak, JR

Published Date

  • 2003

Published In

Volume / Issue

  • 23 / 6

Start / End Page

  • 561 - 567

PubMed ID

  • 14705072

International Standard Serial Number (ISSN)

  • 0738-1085

Digital Object Identifier (DOI)

  • 10.1002/micr.10210

Language

  • eng

Conference Location

  • United States