C1-esterase inhibitor and a novel peptide inhibitor improve contractile function in reperfused skeletal muscle.

Journal Article

To determine the role of inhibition of complement activation in the contractile function of skeletal muscle ischemia-reperfusion (I/R) injury, the rat extensor digitorum longus (EDL) muscles underwent 3 h ischemia and received human C1-esterase inhibitor (C1-INH, 100 IU/kg), a synthetic C1q A chain peptide with a similar inhibitory effect on activated C1 (peptide, 5 mg/kg), or human serum albumin control. Results showed a significant overall increase in tetanic contractile forces of the reperfused EDL in both C1-INH and peptide groups compared to controls. Maximum improvement occurred with peptide treatment at 120-Hz stimulation, with an increase in force from 38 +/- 4% of normal in controls to 52 +/- 4% in peptide-treated rats. There were no significant differences between C1-INH and peptide groups. Plasma C3 and C4 activities were significantly increased in both treated groups, suggesting inhibition of complement activation. Our results suggest that complement activation is involved in I/R injury, and inhibition of complement activation may therefore represent a potential therapeutic approach to reducing or preventing I/R injury.

Full Text

Duke Authors

Cited Authors

  • Toomayan, GA; Chen, L-E; Jiang, H-X; Qi, W-N; Seaber, AV; Frank, MM; Urbaniak, JR

Published Date

  • 2003

Published In

Volume / Issue

  • 23 / 6

Start / End Page

  • 561 - 567

PubMed ID

  • 14705072

Pubmed Central ID

  • 14705072

International Standard Serial Number (ISSN)

  • 0738-1085

Digital Object Identifier (DOI)

  • 10.1002/micr.10210


  • eng

Conference Location

  • United States