NF-kappaB p65 involves in reperfusion injury and iNOS gene regulation in skeletal muscle.

Journal Article

This study investigated the effects of inhibition of NF-kappaB activation on microcirculation and inducible NOS expression in reperfused rat cremaster muscle. The muscle from 16 rats underwent 5-h ischemia and 90-min reperfusion. Each rat received NF-kappaB inhibitor pyrrolidine dithiocarbamate (PDTC, 150 mg/kg) or phosphate-buffered saline 15 min before reperfusion. Results showed that PDTC treatment had a significant overall increase in muscle blood flow during reperfusion. Blood flow more rapidly recovered to and over baseline in the PDTC-treated group than in controls, with a significant difference at 10-30 min and 70-90 min. Expression of iNOS mRNA had a 167-fold increase from normal in controls, but was significantly (P < 0.05) reduced to a 63-fold increase in PDTC-treated muscles. In addition, PDTC treatment significantly (P < 0.05) decreased a reperfusion-induced increase in activated NF-kappaB p65 and nuclear p65 protein. Our results suggest that NF-kappaB is involved in I/R injury and that inhibition of NF-kappaB p65 activation affords protection against I/R injury, perhaps via downregulating expression of iNOS transcription.

Full Text

Duke Authors

Cited Authors

  • Qi, W-N; Chaiyakit, P; Cai, Y; Allen, DM; Chen, L-E; Seaber, AV; Urbaniak, JR

Published Date

  • 2004

Published In

Volume / Issue

  • 24 / 4

Start / End Page

  • 316 - 323

PubMed ID

  • 15274191

International Standard Serial Number (ISSN)

  • 0738-1085

Digital Object Identifier (DOI)

  • 10.1002/micr.20030

Language

  • eng

Conference Location

  • United States