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Intermittent pneumatic compression regulates expression of nitric oxide synthases in skeletal muscles.

Publication ,  Journal Article
Tan, X; Qi, W-N; Gu, X; Urbaniak, JR; Chen, L-E
Published in: J Biomech
2006

This study investigated the effects of intermittent pneumatic compression (IPC) on expression of nitric oxide synthase (NOS) isoforms in compressed (anterior tibialis, AT) and uncompressed (cremaster muscles, CM) skeletal muscles. Following IPC application of 0.5, 1, and 5h on both legs of rats, the endothelial NOS (eNOS) mRNA expression was significantly up-regulated to 1.2-, 1.8, and 2.7-fold from normal, respectively, in both AT and CM, and protein expression increased more than 1.5-fold of normal at each time point. Similarly, neuronal NOS expression was up-regulated, but to a lesser degree. In contrast, inducible NOS expression was significantly and time-dependently down-regulated in both muscles. After IPC cessation, eNOS levels returned to normal in both AT and CM. The results confirm our hypothesis that IPC-induced vasodilation is mediated by regulating expression of NOS isoforms, in particular eNOS, in both compressed and uncompressed skeletal muscles. The results also suggest the importance of precisely characterizing expression of each NOS isoform in tissue pathophysiology.

Duke Scholars

Published In

J Biomech

DOI

ISSN

0021-9290

Publication Date

2006

Volume

39

Issue

13

Start / End Page

2430 / 2437

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Nitric Oxide Synthase
  • Muscle, Skeletal
  • Male
  • Intermittent Pneumatic Compression Devices
  • Gene Expression Regulation, Enzymologic
  • Biomedical Engineering
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
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Tan, X., Qi, W.-N., Gu, X., Urbaniak, J. R., & Chen, L.-E. (2006). Intermittent pneumatic compression regulates expression of nitric oxide synthases in skeletal muscles. J Biomech, 39(13), 2430–2437. https://doi.org/10.1016/j.jbiomech.2005.07.022
Tan, Xiangling, Wen-Ning Qi, Xiaosong Gu, James R. Urbaniak, and Long-En Chen. “Intermittent pneumatic compression regulates expression of nitric oxide synthases in skeletal muscles.J Biomech 39, no. 13 (2006): 2430–37. https://doi.org/10.1016/j.jbiomech.2005.07.022.
Tan X, Qi W-N, Gu X, Urbaniak JR, Chen L-E. Intermittent pneumatic compression regulates expression of nitric oxide synthases in skeletal muscles. J Biomech. 2006;39(13):2430–7.
Tan, Xiangling, et al. “Intermittent pneumatic compression regulates expression of nitric oxide synthases in skeletal muscles.J Biomech, vol. 39, no. 13, 2006, pp. 2430–37. Pubmed, doi:10.1016/j.jbiomech.2005.07.022.
Tan X, Qi W-N, Gu X, Urbaniak JR, Chen L-E. Intermittent pneumatic compression regulates expression of nitric oxide synthases in skeletal muscles. J Biomech. 2006;39(13):2430–2437.
Journal cover image

Published In

J Biomech

DOI

ISSN

0021-9290

Publication Date

2006

Volume

39

Issue

13

Start / End Page

2430 / 2437

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Nitric Oxide Synthase
  • Muscle, Skeletal
  • Male
  • Intermittent Pneumatic Compression Devices
  • Gene Expression Regulation, Enzymologic
  • Biomedical Engineering
  • Animals