Intermittent pneumatic compression regulates expression of nitric oxide synthases in skeletal muscles.

Journal Article (Journal Article)

This study investigated the effects of intermittent pneumatic compression (IPC) on expression of nitric oxide synthase (NOS) isoforms in compressed (anterior tibialis, AT) and uncompressed (cremaster muscles, CM) skeletal muscles. Following IPC application of 0.5, 1, and 5h on both legs of rats, the endothelial NOS (eNOS) mRNA expression was significantly up-regulated to 1.2-, 1.8, and 2.7-fold from normal, respectively, in both AT and CM, and protein expression increased more than 1.5-fold of normal at each time point. Similarly, neuronal NOS expression was up-regulated, but to a lesser degree. In contrast, inducible NOS expression was significantly and time-dependently down-regulated in both muscles. After IPC cessation, eNOS levels returned to normal in both AT and CM. The results confirm our hypothesis that IPC-induced vasodilation is mediated by regulating expression of NOS isoforms, in particular eNOS, in both compressed and uncompressed skeletal muscles. The results also suggest the importance of precisely characterizing expression of each NOS isoform in tissue pathophysiology.

Full Text

Duke Authors

Cited Authors

  • Tan, X; Qi, W-N; Gu, X; Urbaniak, JR; Chen, L-E

Published Date

  • 2006

Published In

Volume / Issue

  • 39 / 13

Start / End Page

  • 2430 - 2437

PubMed ID

  • 16225881

International Standard Serial Number (ISSN)

  • 0021-9290

Digital Object Identifier (DOI)

  • 10.1016/j.jbiomech.2005.07.022


  • eng

Conference Location

  • United States