Maximization of signal derived from cDNA microarrays.

Published

Journal Article

Microarray technology is a powerful tool for generating expression data on a large number of genes simultaneously. However, as for any assay, it must be reproducible to give confidence in the results. Using a classical statistical method--the factorial design of experiments--we have assessed the effects of different experimental factors in our system. Significant effects on signal were seen when the standard components were substituted with a different enzyme, fluorescent label, or RNA purification method. This has led to the implementation of an improved procedure that maximizes signal without affecting the variability of the system, thus increasing the signal-to-noise ratio. In addition, we were able to quantify the variability between microarrays and replicates within microarrays.

Full Text

Duke Authors

Cited Authors

  • Wildsmith, SE; Archer, GE; Winkley, AJ; Lane, PW; Bugelski, PJ

Published Date

  • January 2001

Published In

Volume / Issue

  • 30 / 1

Start / End Page

  • 202 - 208

PubMed ID

  • 11196312

Pubmed Central ID

  • 11196312

Electronic International Standard Serial Number (EISSN)

  • 1940-9818

International Standard Serial Number (ISSN)

  • 0736-6205

Digital Object Identifier (DOI)

  • 10.2144/01301dd04

Language

  • eng