Maximization of signal derived from cDNA microarrays.

Journal Article (Journal Article)

Microarray technology is a powerful tool for generating expression data on a large number of genes simultaneously. However, as for any assay, it must be reproducible to give confidence in the results. Using a classical statistical method--the factorial design of experiments--we have assessed the effects of different experimental factors in our system. Significant effects on signal were seen when the standard components were substituted with a different enzyme, fluorescent label, or RNA purification method. This has led to the implementation of an improved procedure that maximizes signal without affecting the variability of the system, thus increasing the signal-to-noise ratio. In addition, we were able to quantify the variability between microarrays and replicates within microarrays.

Full Text

Duke Authors

Cited Authors

  • Wildsmith, SE; Archer, GE; Winkley, AJ; Lane, PW; Bugelski, PJ

Published Date

  • January 2001

Published In

Volume / Issue

  • 30 / 1

Start / End Page

  • 202 - 208

PubMed ID

  • 11196312

International Standard Serial Number (ISSN)

  • 0736-6205

Digital Object Identifier (DOI)

  • 10.2144/01301dd04


  • eng

Conference Location

  • England