Chondrocytes isolated from mature articular cartilage retain the capacity to form functional gap junctions.

Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.

The distribution, expression, and functionality of gap junctions was examined in bovine chondrocytes (BCs) isolated from mature articular cartilage. BC cells displayed immunoreactivity for connexin 43 (Cx43), a specific gap junction protein. Cx43 protein expression was confirmed by Western blot analysis, and Cx43 mRNA was detected by nuclease protection assay. Additionally, BCs were shown to be functionally coupled, as revealed by dye transfer studies, and octanol, a gap junction uncoupler, greatly attenuated coupling. Furthermore, confocal microscopy of fluo-3 loaded BC cells revealed that deformation-induced cytosolic Ca2+ ion (Ca2+) signals propagated from cell-to-cell via gap junctions. To our knowledge, this is the first evidence suggesting that chondrocytes isolated from adult articular cartilage express functional gap junctions.

Full Text

Duke Authors

Cited Authors

  • Donahue, HJ; Guilak, F; Vander Molen, MA; McLeod, KJ; Rubin, CT; Grande, DA; Brink, PR

Published Date

  • September 1995

Published In

Volume / Issue

  • 10 / 9

Start / End Page

  • 1359 - 1364

PubMed ID

  • 7502708

International Standard Serial Number (ISSN)

  • 0884-0431

Digital Object Identifier (DOI)

  • 10.1002/jbmr.5650100913

Language

  • eng

Citation Source

  • PubMed