Humoral immune response to the entire human immunodeficiency virus envelope glycoprotein made in insect cells.

Journal Article

The human immunodeficiency virus envelope gene was expressed in insect cells by using a Baculovirus expression vector. The protein has an apparent molecular mass of 160 kDa, appears on the surface of infected insect cells, and does not appear to be cleaved to glycoproteins gp120 and gp41. Goats immunized with the 160-kDa protein have high titers of antibody that neutralizes virus infection as measured by viral gene expression or cell cytolysis. In addition, immune sera can block fusion of human immunodeficiency virus-infected cells in culture. Both neutralization and fusion-blocking activities are bound to and eluted from immobilized gp120.

Full Text

Duke Authors

Cited Authors

  • Rusche, JR; Lynn, DL; Robert-Guroff, M; Langlois, AJ; Lyerly, HK; Carson, H; Krohn, K; Ranki, A; Gallo, RC; Bolognesi, DP

Published Date

  • October 1, 1987

Published In

Volume / Issue

  • 84 / 19

Start / End Page

  • 6924 - 6928

PubMed ID

  • 3477816

International Standard Serial Number (ISSN)

  • 0027-8424

Language

  • eng

Conference Location

  • United States