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Active immunization using dendritic cells mixed with tumor cells inhibits the growth of primary breast cancer.

Publication ,  Journal Article
Coveney, E; Wheatley, GH; Lyerly, HK
Published in: Surgery
August 1997

BACKGROUND: Dendritic cells (DCs) are potent antigen-presenting cells regarded as crucial in the priming of an immune response. The goal of our study was to test whether bone marrow-generated DCs are capable of inducing protective immunity against a murine breast carcinoma (4T1). METHODS: DCs were grown from Balb/c mice by culturing lymphocyte-immunodepleted bone marrow in murine granulocyte-macrophage colony-stimulating factor containing medium for 10 days. Balb/c mice (five to eight per group) were immunized intradermally with 1 x 10(6) DCs mixed with 2 x 10(6) lethally irradiated 4T1 cells on day 0. Mice in control groups were given intradermal inoculations of phosphate-buffered saline solution, 1 x 10(6) DCs, or lethally irradiated 4T1 cells alone. Booster intraperitoneal immunizations of 2 x 10(6) lethally irradiated 4T1 cells were given on days 7 and 14. All mice were challenged with 5 x 10(3) 4T1 cells subcutaneously 7 days after the final immunization. Animals were examined daily, and tumor volume was recorded twice weekly with calipers. RESULTS: At 21 days there was a significant reduction in tumor growth in mice immunized with DCs mixed with irradiated 4T1 cells as compared with the control groups (p = 0.0005, Kruskal-Wallis test). CONCLUSIONS: These results suggest that DCs mixed with tumor cells as a source of undefined tumor antigen can induce an effective antitumor immune response. This finding provides a rationale for the use of cultured DCs in immunotherapy of breast and other cancers.

Duke Scholars

Published In

Surgery

DOI

ISSN

0039-6060

Publication Date

August 1997

Volume

122

Issue

2

Start / End Page

228 / 234

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes, Cytotoxic
  • Surgery
  • Phenotype
  • Mice, Inbred BALB C
  • Mice
  • Mammary Neoplasms, Experimental
  • Male
  • Lymphocyte Depletion
  • Immunotherapy, Active
 

Citation

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ICMJE
MLA
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Coveney, E., Wheatley, G. H., & Lyerly, H. K. (1997). Active immunization using dendritic cells mixed with tumor cells inhibits the growth of primary breast cancer. Surgery, 122(2), 228–234. https://doi.org/10.1016/s0039-6060(97)90013-1
Coveney, E., G. H. Wheatley, and H. K. Lyerly. “Active immunization using dendritic cells mixed with tumor cells inhibits the growth of primary breast cancer.Surgery 122, no. 2 (August 1997): 228–34. https://doi.org/10.1016/s0039-6060(97)90013-1.
Coveney, E., et al. “Active immunization using dendritic cells mixed with tumor cells inhibits the growth of primary breast cancer.Surgery, vol. 122, no. 2, Aug. 1997, pp. 228–34. Pubmed, doi:10.1016/s0039-6060(97)90013-1.
Journal cover image

Published In

Surgery

DOI

ISSN

0039-6060

Publication Date

August 1997

Volume

122

Issue

2

Start / End Page

228 / 234

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes, Cytotoxic
  • Surgery
  • Phenotype
  • Mice, Inbred BALB C
  • Mice
  • Mammary Neoplasms, Experimental
  • Male
  • Lymphocyte Depletion
  • Immunotherapy, Active