Identification of envelope V3 loop as the major determinant of CD4 neutralization sensitivity of HIV-1.

Published

Journal Article

Laboratory isolates of human immunodeficiency virus type-1 (HIV-1) such as HTLV-IIIB are generally T cell line-tropic and highly sensitive to neutralization by soluble CD4 (sCD4), a potential antiviral agent that is undergoing clinical trial. However, many primary HIV-1 isolates are macrophage-tropic and sCD4-resistant. Envelope V3 loop sequences derived from primary HIV-1 isolates were sufficient to confer on HTLV-IIIB not only the tissue tropism but also the degree of sCD4 neutralization resistance characteristic of their HIV-1 strains of origin. Single amino acid changes in the V3 loop enhanced sCD4 resistance by up to tenfold. These observations suggest that the tissue tropism and sCD4 neutralization sensitivity of HIV-1 isolates are regulated by similar mechanisms.

Full Text

Duke Authors

Cited Authors

  • Hwang, SS; Boyle, TJ; Lyerly, HK; Cullen, BR

Published Date

  • July 1992

Published In

Volume / Issue

  • 257 / 5069

Start / End Page

  • 535 - 537

PubMed ID

  • 1636088

Pubmed Central ID

  • 1636088

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.1636088

Language

  • eng