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Induction of tumor-specific cytotoxic T lymphocytes in cancer patients by autologous tumor RNA-transfected dendritic cells.

Publication ,  Journal Article
Nair, SK; Morse, M; Boczkowski, D; Cumming, RI; Vasovic, L; Gilboa, E; Lyerly, HK
Published in: Ann Surg
April 2002

OBJECTIVE: To demonstrate the feasibility of inducing tumor antigen-specific immune responses in patients with metastatic cancer using total tumor RNA-loaded dendritic cells (DCs). SUMMARY BACKGROUND DATA: The authors have shown that DCs transfected with mRNA encoding defined tumor antigens induce tumor antigen-specific T-cell responses in vitro and in vivo. There may be significant advantages to inducing immune responses against the entire repertoire of antigens expressed by a patient's autologous tumor. METHODS: RNA was extracted from a metastatic colon cancer and used to load autologous DCs. The DCs were coincubated with autologous T cells and the cytolytic activity of the T cells was assessed by the ability to lyse the autologous tumor cells. RNA was then extracted from a metastatic lung cancer and used to load autologous DCs, followed by four injections of the DC vaccine given every 4 weeks. Tumor antigen-specific cytotoxic T lymphocyte activity was then evaluated by testing peripheral blood mononuclear cells for their ability to lyse an antigen-expressing target. RESULTS: DCs transfected with the total RNA content of autologous tumor cells stimulated antigen-specific T-cell responses that are capable of recognizing and lysing autologous, primary tumor cells in vitro. Tumor-specific immune responses were induced in a patient with a carcinoembryonic antigen-expressing adenocarcinoma after immunization with autologous DCs transfected with total tumor RNA. CONCLUSIONS: DCs transfected with total tumor RNA may represent a method for inducing immune responses against the entire repertoire of tumor antigens of surgically resected malignancies.

Duke Scholars

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Published In

Ann Surg

DOI

ISSN

0003-4932

Publication Date

April 2002

Volume

235

Issue

4

Start / End Page

540 / 549

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • T-Lymphocytes, Cytotoxic
  • Surgery
  • Middle Aged
  • Melanoma
  • Male
  • Lung Neoplasms
  • Liver Neoplasms
  • In Vitro Techniques
 

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Nair, S. K., Morse, M., Boczkowski, D., Cumming, R. I., Vasovic, L., Gilboa, E., & Lyerly, H. K. (2002). Induction of tumor-specific cytotoxic T lymphocytes in cancer patients by autologous tumor RNA-transfected dendritic cells. Ann Surg, 235(4), 540–549. https://doi.org/10.1097/00000658-200204000-00013
Nair, Smita K., Michael Morse, David Boczkowski, R Ian Cumming, Ljiljana Vasovic, Eli Gilboa, and H Kim Lyerly. “Induction of tumor-specific cytotoxic T lymphocytes in cancer patients by autologous tumor RNA-transfected dendritic cells.Ann Surg 235, no. 4 (April 2002): 540–49. https://doi.org/10.1097/00000658-200204000-00013.
Nair SK, Morse M, Boczkowski D, Cumming RI, Vasovic L, Gilboa E, et al. Induction of tumor-specific cytotoxic T lymphocytes in cancer patients by autologous tumor RNA-transfected dendritic cells. Ann Surg. 2002 Apr;235(4):540–9.
Nair, Smita K., et al. “Induction of tumor-specific cytotoxic T lymphocytes in cancer patients by autologous tumor RNA-transfected dendritic cells.Ann Surg, vol. 235, no. 4, Apr. 2002, pp. 540–49. Pubmed, doi:10.1097/00000658-200204000-00013.
Nair SK, Morse M, Boczkowski D, Cumming RI, Vasovic L, Gilboa E, Lyerly HK. Induction of tumor-specific cytotoxic T lymphocytes in cancer patients by autologous tumor RNA-transfected dendritic cells. Ann Surg. 2002 Apr;235(4):540–549.

Published In

Ann Surg

DOI

ISSN

0003-4932

Publication Date

April 2002

Volume

235

Issue

4

Start / End Page

540 / 549

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • T-Lymphocytes, Cytotoxic
  • Surgery
  • Middle Aged
  • Melanoma
  • Male
  • Lung Neoplasms
  • Liver Neoplasms
  • In Vitro Techniques