Preparation of peptide-loaded dendritic cells for cancer immunotherapy.
Publication
, Journal Article
Morse, MA; Clay, T; Colling, K; Lyerly, HK
Published in: Mol Biotechnol
September 2003
Dendritic cell-based vaccines are being evaluated in clinical trials to determine their ability to activate clinically relevant tumor antigen-specific immune responses. Although some groups isolate dendritic cells from peripheral blood, most have found it more efficient to generate large numbers from peripheral blood progenitors, particularly plastic adherent or CD14+ monocytes, in media supplemented with GM-CSF and IL-4. These DC may then be matured, if desired, and loaded with antigen, such as tumor-associated peptides, prior to administration. We describe the scheme that we are currently using to generate peptide-loaded dendritic cells for our clinical trials of cancer immunotherapy.
Duke Scholars
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Published In
Mol Biotechnol
DOI
ISSN
1073-6085
Publication Date
September 2003
Volume
25
Issue
1
Start / End Page
95 / 99
Location
Switzerland
Related Subject Headings
- Tumor Necrosis Factor-alpha
- Tumor Necrosis Factor Receptor-Associated Peptides and Proteins
- TNF Receptor-Associated Factor 3
- Peptides
- Neoplasms
- Leukocytes, Mononuclear
- Leukapheresis
- Interferon-gamma
- Immunotherapy, Active
- Immunotherapy
Citation
APA
Chicago
ICMJE
MLA
NLM
Morse, M. A., Clay, T., Colling, K., & Lyerly, H. K. (2003). Preparation of peptide-loaded dendritic cells for cancer immunotherapy. Mol Biotechnol, 25(1), 95–99. https://doi.org/10.1385/MB:25:1:95
Morse, Michael A., Tim Clay, Kirsten Colling, and H Kim Lyerly. “Preparation of peptide-loaded dendritic cells for cancer immunotherapy.” Mol Biotechnol 25, no. 1 (September 2003): 95–99. https://doi.org/10.1385/MB:25:1:95.
Morse MA, Clay T, Colling K, Lyerly HK. Preparation of peptide-loaded dendritic cells for cancer immunotherapy. Mol Biotechnol. 2003 Sep;25(1):95–9.
Morse, Michael A., et al. “Preparation of peptide-loaded dendritic cells for cancer immunotherapy.” Mol Biotechnol, vol. 25, no. 1, Sept. 2003, pp. 95–99. Pubmed, doi:10.1385/MB:25:1:95.
Morse MA, Clay T, Colling K, Lyerly HK. Preparation of peptide-loaded dendritic cells for cancer immunotherapy. Mol Biotechnol. 2003 Sep;25(1):95–99.
Published In
Mol Biotechnol
DOI
ISSN
1073-6085
Publication Date
September 2003
Volume
25
Issue
1
Start / End Page
95 / 99
Location
Switzerland
Related Subject Headings
- Tumor Necrosis Factor-alpha
- Tumor Necrosis Factor Receptor-Associated Peptides and Proteins
- TNF Receptor-Associated Factor 3
- Peptides
- Neoplasms
- Leukocytes, Mononuclear
- Leukapheresis
- Interferon-gamma
- Immunotherapy, Active
- Immunotherapy