Preparation of peptide-loaded dendritic cells for cancer immunotherapy.

Journal Article (Journal Article)

Dendritic cell-based vaccines are being evaluated in clinical trials to determine their ability to activate clinically relevant tumor antigen-specific immune responses. Although some groups isolate dendritic cells from peripheral blood, most have found it more efficient to generate large numbers from peripheral blood progenitors, particularly plastic adherent or CD14+ monocytes, in media supplemented with GM-CSF and IL-4. These DC may then be matured, if desired, and loaded with antigen, such as tumor-associated peptides, prior to administration. We describe the scheme that we are currently using to generate peptide-loaded dendritic cells for our clinical trials of cancer immunotherapy.

Full Text

Duke Authors

Cited Authors

  • Morse, MA; Clay, T; Colling, K; Lyerly, HK

Published Date

  • September 2003

Published In

Volume / Issue

  • 25 / 1

Start / End Page

  • 95 - 99

PubMed ID

  • 13679640

International Standard Serial Number (ISSN)

  • 1073-6085

Digital Object Identifier (DOI)

  • 10.1385/MB:25:1:95


  • eng

Conference Location

  • United States