Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT.
BACKGROUND: Cryopreservation of PBMC and/or overnight shipping of samples are required for many clinical trials, despite their potentially adverse effects upon immune monitoring assays such as MHC-peptide tetramer staining, cytokine flow cytometry (CFC), and ELISPOT. In this study, we compared the performance of these assays on leukapheresed PBMC shipped overnight in medium versus cryopreserved PBMC from matched donors. RESULTS: Using CMV pp65 peptide pool stimulation or pp65 HLA-A2 tetramer staining, there was significant correlation between shipped and cryopreserved samples for each assay (p
Maecker, HT; Moon, J; Bhatia, S; Ghanekar, SA; Maino, VC; Payne, JK; Kuus-Reichel, K; Chang, JC; Summers, A; Clay, TM; Morse, MA; Lyerly, HK; DeLaRosa, C; Ankerst, DP; Disis, ML
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