Overexpression of TAR sequences renders cells resistant to human immunodeficiency virus replication.

Journal Article

Overexpression of TAR-containing sequences (TAR decoys) was used to render cells resistant to HIV replication. A chimeric tRNA(meti)-TAR transcription unit contained in a double copy murine retroviral vector was used to express high levels of HIV-1 TAR-containing transcripts in CEM SS cells. Replication of HIV-1 was inhibited over 99% in cells expressing chimeric tRNA-TAR transcripts, but an amphotropic murine retrovirus replicated normally in these cells. Expression of TAR sequences in CEM SS cells had no adverse effects on cell viability, indicating that essential cellular factors are not being sequestered in these cells. TAR decoy RNA-mediated HIV inhibition may also be effective against natural HIV isolates in spite of their hypervariable nature, as suggested by the fact that replication of SIVmac was also inhibited in cells expressing HIV-1 TAR decoys.

Full Text

Duke Authors

Cited Authors

  • Sullenger, BA; Gallardo, HF; Ungers, GE; Gilboa, E

Published Date

  • November 2, 1990

Published In

Volume / Issue

  • 63 / 3

Start / End Page

  • 601 - 608

PubMed ID

  • 2225067

International Standard Serial Number (ISSN)

  • 0092-8674

Language

  • eng

Conference Location

  • United States