Overexpression of RRE-derived sequences inhibits HIV-1 replication in CEM cells.
Journal Article (Journal Article)
Overexpression of sequences corresponding to the major Rev-binding site in the Rev response element of human immunodeficiency virus type 1 (HIV-1) (RRE decoys) was used to render cells resistant to HIV-1 replication. This was accomplished by the use of a chimeric tRNA-RRE transcription unit in a double-copy murine retroviral vector to express high levels of HIV-1 RRE-containing transcripts in CEM SS cells. Replication of HIV-1 was inhibited more than 90% in cells expressing chimeric tRNA-RRE transcripts, as determined by in situ immunofluorescence analysis and a p24 antigen ELISA test. Analysis of RNA from HIV-1-infected cells suggests that expression of RRE-containing sequences in CEM SS cells inhibits HIV-1 replication by interfering with Rev function, presumably by competing for Rev binding to its physiological target. The use of a subfragment of RRE as decoy RNA reduces the likelihood that essential cellular factors will be sequestered in cells expressing the decoy RNA. Thus, use of RRE-based decoy RNA to inhibit HIV-1 replication may represent a safer alternative to the use of TAR decoy RNA.
Full Text
Duke Authors
Cited Authors
- Lee, TC; Sullenger, BA; Gallardo, HF; Ungers, GE; Gilboa, E
Published Date
- January 1, 1992
Published In
Volume / Issue
- 4 / 1
Start / End Page
- 66 - 74
PubMed ID
- 1536832
International Standard Serial Number (ISSN)
- 1043-4674
Language
- eng
Conference Location
- United States