Overexpression of RRE-derived sequences inhibits HIV-1 replication in CEM cells.

Journal Article

Overexpression of sequences corresponding to the major Rev-binding site in the Rev response element of human immunodeficiency virus type 1 (HIV-1) (RRE decoys) was used to render cells resistant to HIV-1 replication. This was accomplished by the use of a chimeric tRNA-RRE transcription unit in a double-copy murine retroviral vector to express high levels of HIV-1 RRE-containing transcripts in CEM SS cells. Replication of HIV-1 was inhibited more than 90% in cells expressing chimeric tRNA-RRE transcripts, as determined by in situ immunofluorescence analysis and a p24 antigen ELISA test. Analysis of RNA from HIV-1-infected cells suggests that expression of RRE-containing sequences in CEM SS cells inhibits HIV-1 replication by interfering with Rev function, presumably by competing for Rev binding to its physiological target. The use of a subfragment of RRE as decoy RNA reduces the likelihood that essential cellular factors will be sequestered in cells expressing the decoy RNA. Thus, use of RRE-based decoy RNA to inhibit HIV-1 replication may represent a safer alternative to the use of TAR decoy RNA.

Full Text

Duke Authors

Cited Authors

  • Lee, TC; Sullenger, BA; Gallardo, HF; Ungers, GE; Gilboa, E

Published Date

  • January 1, 1992

Published In

Volume / Issue

  • 4 / 1

Start / End Page

  • 66 - 74

PubMed ID

  • 1536832

International Standard Serial Number (ISSN)

  • 1043-4674

Language

  • eng

Conference Location

  • United States