Scholars@Duke publication: Swine lungs expressing human complement-regulatory proteins are protected against acute pulmonary dysfunction in a human plasma perfusion model.

Swine lungs expressing human complement-regulatory proteins are protected against acute pulmonary dysfunction in a human plasma perfusion model.

Publication , Journal Article

Daggett, CW; Yeatman, M; Lodge, AJ; Chen, EP; Van Trigt, P; Byrne, GW; Logan, JS; Lawson, JH; Platt, JL; Davis, RD

Published in: J Thorac Cardiovasc Surg

February 1997

UNLABELLED: Pulmonary transplantation is currently limited by the number of suitable cadaver donor lungs. For this reason, pulmonary xenotransplantation is currently being investigated. OBJECTIVE: Our goal was to assess the role of complement in pulmonary xenograft dysfunction. METHODS: The pulmonary function of swine expressing human decay accelerating factor and human CD59 (n = 6) was compared with that of the lungs from nontransgenic (control) swine (n = 6) during perfusion with human plasma. RESULTS: After 2 hours of perfusion, the pulmonary vascular resistance was 1624 +/- 408 dynes.sec.cm-5 in control lungs and 908 +/- 68 dynes.sec.cm-5 in transgenic lungs (p < 0.05). Control lungs had a venous oxygen tension of 271 +/- 23 mm Hg with a ratio of venous oxygen tension to inspired oxygen fraction of 452 +/- 38 at 2 hours of perfusion; transgenic lungs had a venous oxygen tension of 398 +/- 11 mm Hg and a ratio of venous oxygen tension to inspired oxygen fraction of 663 +/- 18 (p < 0.05). Control lungs showed a decrease of 79.8% +/- 3.7% in static pulmonary compliance by 2 hours, versus a 12.0% +/- 8.1% decrease by the transgenic lungs (p < 0.05). The control lungs also developed 561.7 +/- 196.2 ml of airway edema over 2 hours, in contrast to 6.5 +/- 1.7 ml in transgenic lungs (p < 0.05). CONCLUSION: Lungs from swine expressing human decay accelerating factor and human CD59 functioned better than nontransgenic swine lungs when perfused with human plasma. These results suggest that complement activation is involved in producing acute pulmonary xenograft dysfunction and demonstrate that lungs from swine expressing human decay accelerating factor and human CD59 are protected against pulmonary injury when perfused with human plasma.

Duke Scholars

Author Edward Po-Chung Chen Surgery, Cardiovascular and Thoracic Surgery

Author Jeffrey Harold Lawson Surgery, Vascular and Endovascular Surgery

Published In

J Thorac Cardiovasc Surg

DOI

10.1016/S0022-5223(97)70337-4

ISSN

0022-5223

Publication Date

February 1997

Volume

113

Issue

Start / End Page

390 / 398

Location

United States

Related Subject Headings

Vascular Resistance
Transplantation, Heterologous
Swine
Respiratory System
Reperfusion
Pulmonary Gas Exchange
Pulmonary Artery
Models, Biological
Microscopy, Fluorescence
Lung Transplantation

Citation

APA

Chicago

ICMJE

MLA

NLM

Daggett, C. W., Yeatman, M., Lodge, A. J., Chen, E. P., Van Trigt, P., Byrne, G. W., … Davis, R. D. (1997). Swine lungs expressing human complement-regulatory proteins are protected against acute pulmonary dysfunction in a human plasma perfusion model. J Thorac Cardiovasc Surg, 113(2), 390–398. https://doi.org/10.1016/S0022-5223(97)70337-4

Daggett, C. W., M. Yeatman, A. J. Lodge, E. P. Chen, P. Van Trigt, G. W. Byrne, J. S. Logan, J. H. Lawson, J. L. Platt, and R. D. Davis. “Swine lungs expressing human complement-regulatory proteins are protected against acute pulmonary dysfunction in a human plasma perfusion model.” J Thorac Cardiovasc Surg 113, no. 2 (February 1997): 390–98. https://doi.org/10.1016/S0022-5223(97)70337-4.

Daggett CW, Yeatman M, Lodge AJ, Chen EP, Van Trigt P, Byrne GW, et al. Swine lungs expressing human complement-regulatory proteins are protected against acute pulmonary dysfunction in a human plasma perfusion model. J Thorac Cardiovasc Surg. 1997 Feb;113(2):390–8.

Daggett, C. W., et al. “Swine lungs expressing human complement-regulatory proteins are protected against acute pulmonary dysfunction in a human plasma perfusion model.” J Thorac Cardiovasc Surg, vol. 113, no. 2, Feb. 1997, pp. 390–98. Pubmed, doi:10.1016/S0022-5223(97)70337-4.

Daggett CW, Yeatman M, Lodge AJ, Chen EP, Van Trigt P, Byrne GW, Logan JS, Lawson JH, Platt JL, Davis RD. Swine lungs expressing human complement-regulatory proteins are protected against acute pulmonary dysfunction in a human plasma perfusion model. J Thorac Cardiovasc Surg. 1997 Feb;113(2):390–398.