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Gene therapy in tissue-engineered blood vessels.

Publication ,  Journal Article
Fields, RC; Solan, A; McDonagh, KT; Niklason, LE; Lawson, JH
Published in: Tissue Eng
December 2003

Cardiovascular disease is the leading cause of morbidity and mortality in Western society. More than 1 million arterial bypass procedures are performed annually in the United States, where either autologous veins or synthetic grafts are used to replace arteries in the coronary or peripheral circulation. Tissue engineering of blood vessels from autologous cells has the potential to produce biological grafts for use in bypass surgery. Ex vivo development of vascular grafts also provides an ideal target of site-specific gene therapy to optimize the physiology of the developing conduit, and for the possible delivery of other therapeutic genes to a vascular bed of interest. In this article, we demonstrate that by using a novel retroviral gene delivery system, a target gene of interest can be specifically delivered to the endothelial cells of a developing engineered vessel. Further, we demonstrate that this technique results in stable incorporation of the delivered gene into the target endothelial cells for more than 30 days. These data demonstrate the utility of the retroviral gene delivery approach for optimizing the biologic phenotype of engineered vessels. This also provides the framework for testing an array of genes that may improve the function of engineered blood vessels after surgical implantation.

Duke Scholars

Published In

Tissue Eng

DOI

ISSN

1076-3279

Publication Date

December 2003

Volume

9

Issue

6

Start / End Page

1281 / 1287

Location

United States

Related Subject Headings

  • Transplants
  • Transfection
  • Tissue Engineering
  • Swine
  • Retroviridae
  • Mice
  • Infusion Pumps, Implantable
  • Humans
  • Genetic Therapy
  • Gene Transfer Techniques
 

Citation

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Fields, R. C., Solan, A., McDonagh, K. T., Niklason, L. E., & Lawson, J. H. (2003). Gene therapy in tissue-engineered blood vessels. Tissue Eng, 9(6), 1281–1287. https://doi.org/10.1089/10763270360728198
Fields, Ryan C., Amy Solan, Kevin T. McDonagh, Laura E. Niklason, and Jeffrey H. Lawson. “Gene therapy in tissue-engineered blood vessels.Tissue Eng 9, no. 6 (December 2003): 1281–87. https://doi.org/10.1089/10763270360728198.
Fields RC, Solan A, McDonagh KT, Niklason LE, Lawson JH. Gene therapy in tissue-engineered blood vessels. Tissue Eng. 2003 Dec;9(6):1281–7.
Fields, Ryan C., et al. “Gene therapy in tissue-engineered blood vessels.Tissue Eng, vol. 9, no. 6, Dec. 2003, pp. 1281–87. Pubmed, doi:10.1089/10763270360728198.
Fields RC, Solan A, McDonagh KT, Niklason LE, Lawson JH. Gene therapy in tissue-engineered blood vessels. Tissue Eng. 2003 Dec;9(6):1281–1287.

Published In

Tissue Eng

DOI

ISSN

1076-3279

Publication Date

December 2003

Volume

9

Issue

6

Start / End Page

1281 / 1287

Location

United States

Related Subject Headings

  • Transplants
  • Transfection
  • Tissue Engineering
  • Swine
  • Retroviridae
  • Mice
  • Infusion Pumps, Implantable
  • Humans
  • Genetic Therapy
  • Gene Transfer Techniques