The footprint of antibody bound to pig cells: evidence of complex surface topology.

Journal Article

The disaccharide Gal(alpha)1-3Gal is found on more than 45 different molecules on the endothelium of porcine cells and has recently attracted considerable interest, being the major target recognized by xenoreactive antibodies. In this study, the distribution and topology of Gal(alpha)1-3Gal on porcine endothelial cells was examined to access whether some Gal(alpha)1-3Gal-containing molecules might be preferentially recognized by antibodies binding to Gal(alpha)1-3Gal. Thirteen percent of the Gal(alpha)1-3Gal was found on glycolipid and 87% on glycoproteins. Of all the glycoproteins and glycolipids containing Gal(alpha)1-3Gal, two molecules, fibronectin and the integrin beta1 subunit, were most intensely labeled by galactose oxidase, suggesting that these molecules may be preferentially exposed on the apical surface of the endothelium. Binding of anti-Gal(alpha)1-3Gal antibodies to endothelial cell surfaces significantly diminished labeling of fibronectin and the integrin beta1 subunit by galactose oxidase, indicating that these glycoproteins are targets for the antibodies when binding to intact porcine cells.

Full Text

Duke Authors

Cited Authors

  • Everett, ML; Lin, SS; Worrell, SS; Platt, JL; Parker, W

Published Date

  • February 14, 2003

Published In

Volume / Issue

  • 301 / 3

Start / End Page

  • 751 - 757

PubMed ID

  • 12565844

International Standard Serial Number (ISSN)

  • 0006-291X

Language

  • eng

Conference Location

  • United States