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Natural anti-carbohydrate IgM in mice: dependence on age and strain.

Publication ,  Journal Article
Love, SD; Lee, W; Nakamura, YC; Platt, JL; Bollinger, RR; Parker, W
Published in: J Immunol Methods
December 1, 2000

Natural anti-carbohydrate antibodies in humans play a key role in natural immunity and in recognition of allogeneic and xenogeneic antigens. Presumably, natural anti-carbohydrate antibodies in mice have similar functions; but these antibodies have not been extensively characterized. An assay was developed and used to screen for anti-carbohydrate IgM in the serum of BDF-1 mice. Among the natural anti-carbohydrate IgM identified, anti-betaGlcNAc IgM were the most abundant. Anti-betaGlcNAc IgG was not detected. Levels of anti-betaGlcNAc IgM were very low in 3-week-old BDF-1 mice and increased until 5 to 7 months of age. Levels of serum anti-betaGlcNAc IgM similar to those in BDF-1 mice were found in the serum of some strains related to the BDF-1 strain (DBA and C57BL/6) and in BDF mice lacking the galactosyl transferase gene. However, in two strains unrelated to the BDF-1 strain (FVB and SJL), levels of anti-betaGlcNAc IgM were less than one-tenth of those found in BDF-1 mice. These results provide considerable insight into the effect of age on the production of natural anti-carbohydrate antibodies in mice and indicate that production of those antibodies is strongly dependent on the strain of mouse. These studies will help in future development of murine models for studying the biological and medical roles of natural anti-carbohydrate antibodies.

Duke Scholars

Published In

J Immunol Methods

DOI

ISSN

0022-1759

Publication Date

December 1, 2000

Volume

246

Issue

1-2

Start / End Page

61 / 68

Location

Netherlands

Related Subject Headings

  • Species Specificity
  • Mice, Inbred DBA
  • Mice, Inbred C57BL
  • Mice, Inbred C3H
  • Mice
  • Immunology
  • Immunoglobulin M
  • Enzyme-Linked Immunosorbent Assay
  • Animals
  • Aging
 

Citation

APA
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MLA
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Love, S. D., Lee, W., Nakamura, Y. C., Platt, J. L., Bollinger, R. R., & Parker, W. (2000). Natural anti-carbohydrate IgM in mice: dependence on age and strain. J Immunol Methods, 246(1–2), 61–68. https://doi.org/10.1016/s0022-1759(00)00296-9
Love, S. D., W. Lee, Y. C. Nakamura, J. L. Platt, R. R. Bollinger, and W. Parker. “Natural anti-carbohydrate IgM in mice: dependence on age and strain.J Immunol Methods 246, no. 1–2 (December 1, 2000): 61–68. https://doi.org/10.1016/s0022-1759(00)00296-9.
Love SD, Lee W, Nakamura YC, Platt JL, Bollinger RR, Parker W. Natural anti-carbohydrate IgM in mice: dependence on age and strain. J Immunol Methods. 2000 Dec 1;246(1–2):61–8.
Love, S. D., et al. “Natural anti-carbohydrate IgM in mice: dependence on age and strain.J Immunol Methods, vol. 246, no. 1–2, Dec. 2000, pp. 61–68. Pubmed, doi:10.1016/s0022-1759(00)00296-9.
Love SD, Lee W, Nakamura YC, Platt JL, Bollinger RR, Parker W. Natural anti-carbohydrate IgM in mice: dependence on age and strain. J Immunol Methods. 2000 Dec 1;246(1–2):61–68.
Journal cover image

Published In

J Immunol Methods

DOI

ISSN

0022-1759

Publication Date

December 1, 2000

Volume

246

Issue

1-2

Start / End Page

61 / 68

Location

Netherlands

Related Subject Headings

  • Species Specificity
  • Mice, Inbred DBA
  • Mice, Inbred C57BL
  • Mice, Inbred C3H
  • Mice
  • Immunology
  • Immunoglobulin M
  • Enzyme-Linked Immunosorbent Assay
  • Animals
  • Aging