Purification and characterization of a 60-kDa protein from oat, formerly known as a TCP1-related chaperone.
Journal Article
Recently, Mummert et al. [Nature 363, 644-648 (1993)] isolated a proposed TCP1-related chaperone. Here we report several findings concerning the protein which they sequenced. Two similar N-terminal sequences were obtained from this abundant 60-kDa protein. Internal sequences were also acquired by protease digestion. Initially it was believed the protein was able to completely inhibit citrate synthase aggregation, but later purifications demonstrated that the 60-kDa polypeptide lacked both chaperone activity and the previously reported kinase activity [Grimm et al., Planta 178, 199-206 (1989)]. It is now our belief that this protein is neither a chaperone nor a kinase.
Full Text
Duke Authors
Cited Authors
- Parker, W; Wells, TA; Meza-Keuthen, S; Kim, IS; Song, PS
Published Date
- February 1995
Published In
Volume / Issue
- 14 / 2
Start / End Page
- 53 - 57
PubMed ID
- 7786406
Pubmed Central ID
- 7786406
International Standard Serial Number (ISSN)
- 0277-8033
Digital Object Identifier (DOI)
- 10.1007/BF01888362
Language
- eng
Conference Location
- United States