Purification and characterization of a 60-kDa protein from oat, formerly known as a TCP1-related chaperone.

Journal Article

Recently, Mummert et al. [Nature 363, 644-648 (1993)] isolated a proposed TCP1-related chaperone. Here we report several findings concerning the protein which they sequenced. Two similar N-terminal sequences were obtained from this abundant 60-kDa protein. Internal sequences were also acquired by protease digestion. Initially it was believed the protein was able to completely inhibit citrate synthase aggregation, but later purifications demonstrated that the 60-kDa polypeptide lacked both chaperone activity and the previously reported kinase activity [Grimm et al., Planta 178, 199-206 (1989)]. It is now our belief that this protein is neither a chaperone nor a kinase.

Full Text

Duke Authors

Cited Authors

  • Parker, W; Wells, TA; Meza-Keuthen, S; Kim, IS; Song, PS

Published Date

  • February 1995

Published In

Volume / Issue

  • 14 / 2

Start / End Page

  • 53 - 57

PubMed ID

  • 7786406

International Standard Serial Number (ISSN)

  • 0277-8033

Language

  • eng

Conference Location

  • United States