Sensitization with xenogeneic tissues alters the heavy chain repertoire of human anti-Galalpha1-3Gal antibodies.

Journal Article

BACKGROUND: Antigen sensitization alters the use of genes encoding the variable and constant regions of immunoglobulin, changing avidity, and function. Alterations in variable region genes induced by carbohydrate antigens have been studied extensively in animals but are incompletely characterized in humans. We asked how sensitization with the carbohydrate Galalpha1-3Gal modifies antibody heavy chain use. METHODS: To overcome limited access to B cells, we analyzed anti-Galalpha1-3Gal antibodies from the serum of naïve and sensitized human subjects with anti-sera specific for VH families. RESULTS: We find that in preimmune subjects, heavy chains of IgM anti-Galalpha1-3Gal derived primarily from VH3 family members, whereas the heavy chains of IgG are from diverse VH families. After sensitization, heavy chains of IgM and IgG antibodies both derived from diverse VH families. CONCLUSIONS: The preimmune repertoire of IgM antibodies to Galalpha1-3Gal is thus more restricted than the antibody repertoire after sensitization, suggesting an antigen-induced shift in the repertoire.

Full Text

Duke Authors

Cited Authors

  • Yu, PB; Parker, W; Nayak, JV; Platt, JL

Published Date

  • July 15, 2005

Published In

Volume / Issue

  • 80 / 1

Start / End Page

  • 102 - 109

PubMed ID

  • 16003240

International Standard Serial Number (ISSN)

  • 0041-1337

Language

  • eng

Conference Location

  • United States